Genome-wide identification and expression analysis of sucrose synthase genes in allotetraploid Brassica juncea

Abstract

Sucrose plays pivotal role in energy metabolism and regulating gene expression of several physiological processes in higher plants. Here, fourteen sucrose synthase (SUS) genes have been identified in the allotetraploid genome of Indian mustard, Brassica juncea. The identified SUS genes in B. juncea (BjSUS) were derived from the two-progenitor species, B. rapa and B. nigra. Intron-exon analysis indicated loss or gain of 1-3 introns in diversification of SUS gene family. Phylogenetic analysis revealed discrete evolutionary paths for the BjSUS genes, originating from three ancestor groups, SUS I, SUS II and SUS III. Gene expression study revealed significant variability in expression of the BjSUS paralogs across the different tissues. BjSUS genes showed transcriptional activation in response to defense hormones and a late response to wounding. Tissue and temporal specificity of expression revealed importance of specific SUS paralogs at different developmental stages and under different stress conditions. The study highlighted differential involvement of SUS paralogs in sucrose metabolism across the tissues and stress-responses, in a major oilseed crop B. juncea.

Keywords: Brassica juncea; Gene structure; SUS family; SUS genes; Sucrose synthase.