Indirect calorimetry is a tool used routinely by sport/exercise physiologist to assess the metabolic response to training and to nutritional interventions. There are different stoichiometric equations to estimate fat (FatOxR ) and carbohydrates (CHOOxR ) oxidation rates, however there are not enough information in literature about what are the most accurate equations. The purpose of this study was to determine the concordance between indirect calorimetry and a method of reference for stoichiometric equations used to estimate FatOxR and CHOOxR . Concordance between indirect calorimetry and the method of reference (13 C to 12 C ratio (13 C:12 C ratio) technique) for key stoichiometric equations was assessed in well-trained triathletes. Subjects carried out a carbohydrate depletion-repletion protocol, labeling the glycogen stores with 13 C, and a laboratory test to assess the 13 C metabolic response during a wide range of aerobic intensities during exercise. All the equations showed a narrow agreement interval (Δ) (CHOOxR nPC (protein component negligible): -0.308, 0.308, CHOOxR PC (protein component): -0.268, 0.268, FatOxR nPC and PC: -0.032, 0.032 (g·min-1 )). FatOxR showed a similar concordance (28-32%) with CHOOxR nPC ranging from 55% to 75%, and for CHOOxR PC between 51% to 71%. None of the stoichiometric equations met a perfect agreement with the method of reference. The Jeukendrup and Wallis equation showed the best concordance for CHOOxR nPC whilst the Frayn and Ferrannini (Glu) equations had the best agreement for CHOOxR PC. All FatOxR equations showed similar concordances and they are able to be used indistinctly.
Keywords: 13C labelled; Method of reference; Non-metabolic CO2; Stoichiometric equations.
© 2019 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of The Physiological Society and the American Physiological Society.