Copper Chaperone for Superoxide Dismutase Promotes Breast Cancer Cell Proliferation and Migration via ROS-Mediated MAPK/ERK Signaling

Yanping Li; Ronghui Liang; Xiaoya Zhang; Jiyan Wang; Changliang Shan; Shuangping Liu; Leilei Li; Shuai Zhang

doi:10.3389/fphar.2019.00356

Copper Chaperone for Superoxide Dismutase Promotes Breast Cancer Cell Proliferation and Migration via ROS-Mediated MAPK/ERK Signaling

Front Pharmacol. 2019 Apr 5:10:356. doi: 10.3389/fphar.2019.00356. eCollection 2019.

Authors

Yanping Li¹, Ronghui Liang¹, Xiaoya Zhang¹, Jiyan Wang², Changliang Shan^{1

2}, Shuangping Liu³, Leilei Li¹, Shuai Zhang⁴

Affiliations

¹ Biomedical Translational Research Institute, Jinan University, Guangzhou, China.
² State Key Laboratory of Medicinal Chemical Biology, College of Pharmacy and Tianjin Key Laboratory of Molecular Drug Research, Nankai University, Tianjin, China.
³ Department of Pathology, Medical School, Dalian University, Dalian, China.
⁴ School of Integrative Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, China.

Abstract

Copper chaperone for superoxide dismutase (CCS) is a critical component of oxidation-reduction system and functions as a potential tumor promoter in several cancers. However, the function and clinical significance of CCS in breast cancer remain unclear. Here, we found CCS was highly expressed in breast cancer, where it promoted breast cancer cell proliferation and migration. Suppression of CCS expression was sufficient to attenuate the phosphorylation level of ERK1/2 and increase the accumulation of reactive oxygen species (ROS). Mechanistically, we found that knockdown of CCS decreases the activity of ERK1/2 mediated by the accumulation of ROS, which leads to the inhibition of cell proliferation and migration. In summary, these results indicated that CCS promotes the growth and migration of breast cancer cells via regulating the ERK1/2 activity mediated by ROS.

Keywords: CCS; MAPK/ERK; ROS; breast cancer; migration; proliferation.