DNA extraction from soil by EZ1 advanced XL (Qiagen)

Forensic Sci Int. 2019 Jun:299:161-167. doi: 10.1016/j.forsciint.2019.04.004. Epub 2019 Apr 12.

Abstract

EZ1 Advanced XL (Qiagen) is an instrument designed to purify automatically nucleic acids from a wide variety of samples: different protocols for DNA extraction from forensic samples are pre-loaded into special EZ1 Advanced XL DNA Investigator Cards. The present study focuses on DNA extraction from biological samples (blood, saliva, sperm) mixed with 3 different types of soils (loam, silt, sandy), using a modified version of the Manufacturer standard protocol. In order to create several traces in the soil, we used biological samples (blood, saliva, sperm) from known donors. Quantification data, reliability and trends in STRs typing success rates using two different commercial multiplexes were evaluated. EZ1 modified DNA extraction protocol allows to recover DNA free of inhibitors and in good quantity for downstream applications.

Keywords: DNA extraction; Mixed samples; Soil.

MeSH terms

  • Blood Chemical Analysis
  • DNA / isolation & purification*
  • DNA Fingerprinting
  • Forensic Genetics / instrumentation*
  • Forensic Genetics / methods
  • Humans
  • Male
  • Microsatellite Repeats
  • Polymerase Chain Reaction
  • Reproducibility of Results
  • Saliva / chemistry
  • Semen / chemistry
  • Soil / chemistry*
  • Specimen Handling

Substances

  • Soil
  • DNA