Abstract
We assessed changes of olfactory bulbs in rata with 6-hydroxydopamine destruction of the substantia nigra. The expression of marker proteins of immature and differentiated neurons and glia (vimentin, PSA-NCAM, tyrosine hydroxylase, and S100) was analyzed by immunohistochemical and morphometric methods. The number of periglomerular dopamine neurons and astroglia in the olfactory bulbs increased on the side of toxin injection and expression of PSA-NCAM and vimentin increased in the rostral migratory stream. Destruction of the substantia nigra shifted differentiation of neuronal progenitors towards the dopaminergic phenotype and increased their survival in the olfactory bulbs, which can be explained by increased expression of PSA-NCAM.
Keywords:
6-hydroxydopamine; PSA-NCAM; olfactory bulbs; rostral migratory stream; tyrosine hydroxylase.
MeSH terms
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Adaptation, Physiological
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Animals
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Biomarkers / metabolism
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Disease Models, Animal
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Gene Expression
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Humans
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Immunohistochemistry
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Injections, Intraventricular
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Male
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Motor Activity / physiology
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Neural Cell Adhesion Molecule L1 / genetics
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Neural Cell Adhesion Molecule L1 / metabolism
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Neuroglia / metabolism
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Neuroglia / pathology*
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Neurons / metabolism
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Neurons / pathology*
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Olfactory Bulb / metabolism
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Olfactory Bulb / pathology*
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Oxidopamine / administration & dosage
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Parkinson Disease, Secondary / chemically induced
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Parkinson Disease, Secondary / genetics
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Parkinson Disease, Secondary / metabolism
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Parkinson Disease, Secondary / pathology*
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Rats
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Rats, Wistar
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S100 Proteins / genetics
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S100 Proteins / metabolism
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Sialic Acids / genetics
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Sialic Acids / metabolism
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Stereotaxic Techniques
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Substantia Nigra / metabolism
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Substantia Nigra / pathology*
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Tyrosine 3-Monooxygenase / genetics
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Tyrosine 3-Monooxygenase / metabolism
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Vimentin / genetics
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Vimentin / metabolism
Substances
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Biomarkers
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Neural Cell Adhesion Molecule L1
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S100 Proteins
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Sialic Acids
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Vimentin
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polysialyl neural cell adhesion molecule
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Oxidopamine
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Tyrosine 3-Monooxygenase