Labeling Peptide Nucleic Acids with Indium-111

Igor G Panyutin

doi:10.1007/978-1-4939-9216-4_12

Labeling Peptide Nucleic Acids with Indium-111

Methods Mol Biol. 2019:1973:185-191. doi: 10.1007/978-1-4939-9216-4_12.

Author

Igor G Panyutin¹

Affiliation

¹ Department of Radiology and Imaging Sciences, Clinical Center, National Institutes of Health, Bethesda, MD, USA. igor.panyutin@nih.gov.

PMID: 31016703
DOI: 10.1007/978-1-4939-9216-4_12

Abstract

Peptide nucleic acids (PNA) are widely used DNA mimics that bind sequence specifically to single- and double-stranded nucleic acids. Hence they are of interest in the design of gene-targeted radiotherapeutics that could deliver radiodamage to designated DNA and/or RNA sites. Here I describe a procedure for incorporation of gamma-emitting radionuclide ¹¹¹In into PNA oligomers. Diethylenetriaminepentaacetic acid (DTPA) was conjugated to a lysine-containing mixed-base PNA. ¹¹¹In-labeled PNAs were obtained by chelation of PNA-DTPA conjugates with ¹¹¹In³⁺ in an acidic aqueous solution.

Keywords: 111In; DNA damage; DTPA; Gene targeting; Peptide nucleic acids.

Publication types

Research Support, N.I.H., Intramural

MeSH terms

Indium Radioisotopes / chemistry*
Isotope Labeling / methods*
Peptide Nucleic Acids / chemistry*

Substances

Indium Radioisotopes
Peptide Nucleic Acids
Indium-111