Non-contiguous finished genome sequencing and description of Enterococcus timonensis sp. nov. isolated from human sputum

M D Mbogning Fonkou; M Bilen; N Gouba; S Khelaifia; F Cadoret; T T Nguyen; M Richez; F Bittar; P-E Fournier; D Raoult; G Dubourg

doi:10.1016/j.nmni.2019.100532

Non-contiguous finished genome sequencing and description of Enterococcus timonensis sp. nov. isolated from human sputum

New Microbes New Infect. 2019 Mar 21:29:100532. doi: 10.1016/j.nmni.2019.100532. eCollection 2019 May.

Authors

M D Mbogning Fonkou¹, M Bilen¹, N Gouba², S Khelaifia¹, F Cadoret¹, T T Nguyen¹, M Richez¹, F Bittar¹, P-E Fournier³, D Raoult^{1

4}, G Dubourg¹

Affiliations

¹ MEPHI, UMR, IRD, Aix-Marseille Univ, Marseille, France, Facultés de Médecine et de Pharmacie, Marseille, France.
² Institut Supérieur des Sciences de La Santé (INSSA), Université Polytechnique de Bobo Dioulasso, Burkina Faso.
³ Aix Marseille Univ, IRD, VITROME, IHU - Méditerranée Infection, 19-21 Boulevard Jean Moulin, 13005, Marseille, France.
⁴ Special Infectious Agents Unit, King Fahd Medical Research Center, King Abdulaziz University, Jeddah, Saudi Arabia.

Abstract

Enterococcus timonensis sp. nov., strain Marseille-P2817^T, is a facultatively anaerobic, motile and non-spore-forming Gram-positive coccus which was isolated from the sputum of a healthy adult man in Marseilles. We present herein its phenotypic description together with MALDI-TOF (matrix-assisted laser-desorption/ionization time-of-flight) mass spectrometry analysis and genome sequencing and comparison. The genome of Enterococcus timonensis is 2 123 933 bp long with 38.46 mol% of G+C content, and it contains 1983 protein-coding genes and 65 RNA genes (including nine rRNA genes).

Keywords: Culturomics; Enterococcus timonensis; genome; sputum; taxono-genomics.