Abstract
This article describes a method for the biofabrication of skin tissue equivalents in a multiwell plate format. The technique and results overcome shortcomings of previously published engineering methods, and show good architecture and barrier function from well to well; thus it may be used for compound functional testing and for the development of disease tissue models for screening.
Keywords:
barrier function; bioprinting; high-throughput screening; keratinocytes; skin.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, N.I.H., Intramural
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Bioprinting*
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Cell Survival / drug effects
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Electric Conductivity
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Epidermis / metabolism
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Humans
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Hydrogels / pharmacology
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Imaging, Three-Dimensional
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Laminin / metabolism
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Membrane Glycoproteins / metabolism
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Printing, Three-Dimensional*
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Reproducibility of Results
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Skin, Artificial*
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Tissue Scaffolds / chemistry*
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Tissue Survival / drug effects
Substances
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Hydrogels
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Laminin
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Membrane Glycoproteins
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nidogen