Development of an enzyme-linked immunosorbent assay and a dipstick assay for the rapid analysis of trans-resveratrol in grape berries

Abstract

trans-Resveratrol, a natural polyphenol present in grapes, has many beneficial effects to human health. However, measurement of trans-resveratrol is technically complicated and time-consuming. In the present study, we obtained a sensitive and specific monoclonal antibody (mAb), namely 3C9, against trans-resveratrol. An indirect competitive enzyme-linked immunosorbent assay (icELISA) was developed, with 50% inhibitory concentration and working range of 1.0 ng/mL and 0.19-4.9 ng/mL, respectively. A lateral flow immunoassay (LFIA) based on 3C9 was also developed for the semiquantitative detection of trans-resveratrol in an indicator range of 50-100 ng/mL. Average recoveries of trans-resveratrol spiked in red and green grape berries samples were respectively 88-107% and 83-102% by icELISA. The icELISA and LFIA were applied for determination of trans-resveratrol in grape berries. The results were highly consistent with those determined by high-performance liquid chromatography analysis (R² = 0.9997). Therefore, we conclude that the immunoassay methods are suitable for the large-scale screening of trans-resveratrol in grape quality breeding.

Keywords: 4-Vinylphenol (Compound CID: 62453); Catechin (Compound CID: 9064); Diethylstilbestrol (Compound CID: 448537); Enzyme-linked immunosorbent assay; Grape berries; Lateral flow immunoassay; Polyphenol; Resorcinol (Compound CID: 5054); cis-Resveratrol (Compound CID: 1548910); trans-Piceid (Compound CID: 5281718); trans-Resveratrol; trans-Resveratrol (Compound CID: 445154).