A mussel-inspired carboxymethyl cellulose hydrogel with enhanced adhesiveness through enzymatic crosslinking

Yujie Zhong; Jie Wang; Zhenyu Yuan; Yu Wang; Zhenhao Xi; Li Li; Zhiyong Liu; Xuhong Guo

doi:10.1016/j.colsurfb.2019.03.044

A mussel-inspired carboxymethyl cellulose hydrogel with enhanced adhesiveness through enzymatic crosslinking

Colloids Surf B Biointerfaces. 2019 Jul 1:179:462-469. doi: 10.1016/j.colsurfb.2019.03.044. Epub 2019 Mar 21.

Authors

Yujie Zhong¹, Jie Wang², Zhenyu Yuan¹, Yu Wang¹, Zhenhao Xi³, Li Li¹, Zhiyong Liu⁴, Xuhong Guo⁵

Affiliations

¹ State Key Laboratory of Chemical Engineering, East China University of Science and Technology, Shanghai, 200237, China.
² State Key Laboratory of Chemical Engineering, East China University of Science and Technology, Shanghai, 200237, China. Electronic address: jiewang2010@ecust.edu.cn.
³ Shanghai Key Laboratory of Multiphase Materials Chemical Engineering, East China University of Science and Technology, Shanghai, 200237, China. Electronic address: zhhxi@ecust.edu.cn.
⁴ Engineering Research Center of Materials Chemical Engineering of Xinjiang Bingtuan, Key Laboratory of Materials Chemical Engineering of Xinjiang Uygur Autonomous Region, Shihezi University, Shihezi, 832000, China.
⁵ State Key Laboratory of Chemical Engineering, East China University of Science and Technology, Shanghai, 200237, China; Engineering Research Center of Materials Chemical Engineering of Xinjiang Bingtuan, Key Laboratory of Materials Chemical Engineering of Xinjiang Uygur Autonomous Region, Shihezi University, Shihezi, 832000, China. Electronic address: guoxuhong@ecust.edu.cn.

PMID: 31005741
DOI: 10.1016/j.colsurfb.2019.03.044

Abstract

In order to replace conventional sutures in wound closing applications, favorable hydrogels with strong wet tissue adhesion and biocompatibility have attracted considerable attention. Herein, inspired by mussel adhesive protein, a series of dopamine modified carboxymethyl cellulose (CMC-DA) hydrogels were prepared in situ using enzymatic crosslinking in the presence of horseradish peroxidase (HRP) and H₂O₂. The biomimetic CMC-DA hydrogel exhibited about 6-fold enhanced wet tissue adhesion strength (28.5 kPa) over the commercial fibrin glue. In addition, the gelation time, swelling ratio and rheological property of the hydrogel can be simply controlled by changing the concentrations of HRP, H₂O₂, and CMC-DA polymer. The gels also exhibited good biodegradation and biocompatibility in vitro. The overall results show that the CMC-DA hydrogel with enhanced wet adhesiveness will be a promising tissue adhesive material.

Keywords: Carboxymethyl cellulose; Enzymatic crosslinking; Mussel-inspired; Tissue adhesive.

MeSH terms

Adhesiveness
Animals
Bivalvia / chemistry*
Carboxymethylcellulose Sodium / chemical synthesis
Carboxymethylcellulose Sodium / chemistry*
Cell Death
Cell Line
Cell Survival
Cross-Linking Reagents / chemistry*
Dopamine
Fibroblasts / metabolism
Horseradish Peroxidase / metabolism*
Hydrogels / chemistry*
Materials Testing*
Mice
Rheology
Swine
Time Factors
Tissue Adhesions / pathology

Substances

Cross-Linking Reagents
Hydrogels
Horseradish Peroxidase
Carboxymethylcellulose Sodium
Dopamine