Association of CAST gene polymorphism with carcass value and meat quality in two synthetic lines of sheep

M Greguła-Kania; T M Gruszecki; A Junkuszew; E Juszczuk-Kubiak; M Florek

doi:10.1016/j.meatsci.2019.04.007

Association of CAST gene polymorphism with carcass value and meat quality in two synthetic lines of sheep

Meat Sci. 2019 Aug:154:69-74. doi: 10.1016/j.meatsci.2019.04.007. Epub 2019 Apr 9.

Authors

M Greguła-Kania¹, T M Gruszecki², A Junkuszew³, E Juszczuk-Kubiak⁴, M Florek⁵

Affiliations

¹ Institute of Animal Breeding and Biodiversity Conservation, University of Life Sciences in Lublin, Akademicka 13, 20-950 Lublin, Poland.
² Institute of Animal Breeding and Biodiversity Conservation, University of Life Sciences in Lublin, Akademicka 13, 20-950 Lublin, Poland. Electronic address: tomasz.gruszecki@up.lublin.pl.
³ Institute of Animal Breeding and Biodiversity Conservation, University of Life Sciences in Lublin, Akademicka 13, 20-950 Lublin, Poland. Electronic address: andrzej.junkuszew@up.lublin.pl.
⁴ Warsaw University of Life Sciences - SGGW, Faculty of Veterinary Medicine, Department of Large Animal Diseases with Clinic, Research Center for Veterinary Medicine, Nowoursynowska 100, 02-787 Warsaw, Poland; Institute of Genetics and Animal Breeding of the Polish Academy of Science, Department of Molecular Biology, Laboratory of Genome and Transcriptome Sequencing, Postępu 36A, Jastrzębiec, 05-552 Magdalenka, Poland. Electronic address: edyta_juszczuk_kubiak@sggw.pl.
⁵ Department of Commodity Science and Processing of Raw Animal Materials, University of Life Sciences in Lublin, Akademicka 13, 20-950 Lublin, Poland. Electronic address: mariusz.florek@up.lublin.pl.

PMID: 31004942
DOI: 10.1016/j.meatsci.2019.04.007

Abstract

The aim of study was to estimate genotype frequencies of the polymorphism in the intron 12 of CAST gene of growing lambs, and to evaluate associations between the CAST genotype and carcass and meat traits. A total number of 317 rams representing the synthetic BCP (163) and SCP (154) meaty lines were genotyped by the RFLP/MspI/NcoI/Hin6I. Three genotypes including "aa", "ae" and "ac" with most frequencies of 0.48, 0.27 and 0.19, were observed in whole population. Lambs with the "aa" CAST genotype showed the highest muscle percentage and the lowest fat percentage in the hind legs. On the other hand, the loins of rams with "ac" genotype had the highest intramuscular fat content, indicative of beneficial health-related properties and technological usefulness. However, differences between CAST genotype stated for the sensory properties, texture and thawing and cooking losses of the meat were insignificant. Breeding efforts aimed at the improvement of lamb meat quality should prioritize the selection of the "ac" genotype.

Keywords: CAST gene; Carcass value; Genetic marker; Lambs; Meat quality.

MeSH terms

Adipose Tissue
Animals
Body Composition / genetics*
Breeding
Calcium-Binding Proteins / genetics*
Cooking
Male
Muscle, Skeletal
Polymorphism, Genetic
Red Meat / analysis*
Sheep, Domestic / genetics

Substances

Calcium-Binding Proteins
calpastatin