Neotropical wild rodents Akodon and Oligoryzomys (Cricetidae: Sigmodontinae) as important carriers of pathogenic renal Leptospira in the Atlantic forest, in Brazil

Abstract

Leptospirosis is an important worldwide zoonosis, caused by a bacterium of the genus Leptospira. For a better understanding of the disease, it is relevant the application of the one health concept. The Atlantic Forest is considered a biodiversity hotspot, with a great endemism of species and despite its importance and proximity to urban areas, the potential role of its fauna as carriers of infectious agents is still poorly understood. Although it is well-known that rats and mice are key reservoirs of leptospires, particularly Rattus norvegicus, wild rodents have also been revealed as reservoirs of leptospiral strains. Thus, the purpose of this study was to investigate the role of the sigmodontine rodents from genera Akodon and Oligoryzomys as pathogenic Leptospira carriers in the Atlantic Forest. We studied 52 Akodon spp. and 15 Oligoryzomys spp. from three areas. Overall 30% were PCR-positive for pathogenic Leptospira, 27% (14/52) Akodon spp. and 40% (6/15) Oligoryzomys spp. DNA sequencing of LipL32 gene confirmed nine species as pathogenic Leptospira. This remarkable incidence of leptospiral carriage within wild genera emphasizes the role of these rodents as carriers of leptospires throughout in this environment.

Keywords: Brazil; Leptospirosis; Molecular diagnosis; One health.