Effects of dietary hydrolyzed yeast (Rhodotorula mucilaginosa) on growth performance, immune response, antioxidant capacity and histomorphology of juvenile Nile tilapia (Oreochromis niloticus)

Abstract

An 8 weeks feeding experiment was conducted to evaluate the effects of dietary supplementation with hydrolyzed yeast (HY) (Rhodotorula mucilaginosa) on growth performance, hematological parameters, immune response and antioxidant ability of juvenile Nile tilapia. Five isonitrogenous and isolipidic diets (32% protein and 4% lipid) with different levels (0%, 0.125%, 0.25%, 0.5%, 1%) of HY were formulated. Each diet was randomly assigned to quadruplicate groups of fish (initial body weight 19.1 ± 0.01 g). Results indicated that significantly higher specific growth rate (SGR) and lower feed conversion rate (FCR) were obtained in fish fed 1% HY diet than that of fish fed 0% HY diet (P < 0.05). Fish fed 0.25% HY diet showed the lowest value of hepatopancreas somatic indices (HSI) and significantly lower than that of fish fed 0% HY diet (P < 0.05). Meanwhile, protein and ash in the whole-body content of fish fed 1% HY diet was significantly higher than that of fish fed 0%-0.5% HY diets. Serum immunological parameters showed that the lysozyme (LZM) activity and Complement C3 content were significantly increased by dietary supplementation of 0.5%-1% HY (P < 0.05). However, dietary supplementation with 0.125%-1% HY significantly decreased the activity of myeloperoxidase (MPO) (P < 0.05). Antioxidant status in serum and liver was significantly enhanced by dietary supplementation of 0.25%-1% HY through the remarkably improved superoxide dismutase (SOD) activity both in serum and liver, the raised total antioxidative capacity (T-AOC) of serum as well as the notably reduced malondialdehyde (MDA) content in the liver (P < 0.05). However, T-AOC in the liver was not significantly influenced among all diet treatments (P > 0.05). Villi height and intraepithelial lymphocytes (IEFs) of mid-intestine were significantly higher in fish fed 0.5%-1% HY diets (P < 0.05). The challenge test demonstrated the enhanced protection against Streptococcus iniae strain by the obtained higher cumulative survival rate. In conclusion, dietary supplementation of 1% HY could maintain the better growth performance, nutrient composition as well as immune response and antioxidant capacity for juvenile Nile tilapia.

Keywords: Histomorphology; Hydrolyzed yeast; Immune response and antioxidant capacity; Rhodotorula mucilaginosa.