Alumina nanoparticles size and crystalline phase impact on cytotoxic effect on alveolar epithelial cells after simple or HCl combined exposures

Abstract

Applications using alumina nanoparticles (Al₂O₃ NPs) have incredibly increased in different fields of activity. In defense and aerospace fields, solid composite propellants use leads to complex combustion aerosols emissions containing high concentrations of Al₂O₃ NPs and hydrogen chloride gas (HCl). To better characterize potential hazard resulting from exposure to these aerosols, this study assesses cytotoxic effects of mixtures containing both compounds on human pulmonary alveolar epithelial cells (A549 cell line) after 24 h exposures. After all co-exposures cell viability was >80%. However co-exposures decrease normalized real-time cell index. Significant decreases of intracellular reduced glutathione pool were also observed after co-exposures to γ-10 nm or γ/δ-13 nm Al₂O₃ NPs and HCl. Co-incubations with γ/δ-13 nm or γ-500 nm Al₂O₃ particles and HCl induced significant DNA double-strand breaks increases. Moreover all co-exposures and HCl alone disrupted cell cycle (increased G1 phase cells). Transmission Electron Microscopy (TEM) observations revealed γ/δ-13 nm Al₂O₃NPs adsorption and internalization in cell cytoplasm only, suggesting indirect genotoxic effects. According to our results Al₂O₃ particles/HCl mixtures can induce cytotoxic effects and Al₂O₃ size and crystallinity are two main parameters influencing cytotoxic mechanisms.

Keywords: Alumina; Alveolar epithelial cells (A549); Genotoxicity; Hydrochloric acid; Nanoparticles; Oxidative stress.