Assessment of the Performance of Solid Phase Extraction Based on Pipette Tip Employing a Hybrid Molecularly Imprinted Polymer as an Adsorbent for Enantioselective Determination of Albendazole Sulfoxide

Sara da Silva Anacleto; Hanna Leijoto de Oliveira; Anny Talita Maria da Silva; Tienne Aparecida do Nascimento; Marcella Matos Cordeiro Borges; Ricky Cássio Dos Santos Silva; Arnaldo César Pereira; Keyller Bastos Borges

doi:10.1093/chromsci/bmz036

Assessment of the Performance of Solid Phase Extraction Based on Pipette Tip Employing a Hybrid Molecularly Imprinted Polymer as an Adsorbent for Enantioselective Determination of Albendazole Sulfoxide

J Chromatogr Sci. 2019 Aug 1;57(7):671-678. doi: 10.1093/chromsci/bmz036.

Authors

Sara da Silva Anacleto¹, Hanna Leijoto de Oliveira¹, Anny Talita Maria da Silva¹, Tienne Aparecida do Nascimento¹, Marcella Matos Cordeiro Borges¹, Ricky Cássio Dos Santos Silva¹, Arnaldo César Pereira¹, Keyller Bastos Borges¹

Affiliation

¹ Departamento de Ciências Naturais, Universidade Federal de São João del-Rei, Campus Dom Bosco, Praça Dom Helvécio 74, Fábricas, 36301-160, São João del-Rei, Minas Gerais, Brazil.

PMID: 31004427
DOI: 10.1093/chromsci/bmz036

Abstract

Herein, an organic-inorganic hybrid molecularly imprinted polymer (MIP) was successfully synthesized with albendazole sulfoxide (ABZSO) as a template and 3-(trimethoxysilyl)propyl methacrylate, a bifunctional group compound, as a single cross-linking agent. In this study, a simple method using HPLC-DAD was developed for the determination of ABZSO enantiomers in human urine using pipette tip-based molecularly imprinted polymer solid phase extraction (PT-MIP-SPE). Enantioseparation with satisfactory retention times (5.17 and 7.09 min), acceptable theoretical plates (N = 4,535 and 5,091) and strong resolution (Rs = 5.45) was performed with an Agilent® Eclipse Plus C18 (100 mm × 4.6 mm, 3.5 μm) coupled with a Chiralpak® IA column (100 mm × 4.6 mm, 3 μm), a mixture with ethanol:water (50:50, v/v) as the mobile phase, temperature at 40°C, flow rate at 0.9 mL min-1 and λ = 230 nm. Thereafter, certain parameters affecting the PT-MIP-SPE were investigated in detail and the better conditions were: 300 μL of water as washing solvent, 500 μL of ethanol:acetic acid (9:1, v/v) as eluting solvent, 20 mg of MIP, 500 μL of human urine at pH 9 and no addition of NaCl. Recoveries/relative standard deviation (RSD%) for (R)-(+)-ABZSO and (S)-(-)-ABZSO were 78.2 ± 0.2% and 69.7 ± 1.7%, respectively.