High efficiency biotransformation of bisphenol A in a fluidized bed reactor using stabilized laccase in porous silica

Mingyue Piao; Donglei Zou; Xianghao Ren; Song Gao; Chuanyu Qin; Yunxian Piao

doi:10.1016/j.enzmictec.2019.03.006

High efficiency biotransformation of bisphenol A in a fluidized bed reactor using stabilized laccase in porous silica

Enzyme Microb Technol. 2019 Jul:126:1-8. doi: 10.1016/j.enzmictec.2019.03.006. Epub 2019 Mar 28.

Authors

Mingyue Piao¹, Donglei Zou², Xianghao Ren³, Song Gao², Chuanyu Qin⁴, Yunxian Piao⁵

Affiliations

¹ Key Laboratory of Groundwater Resources and Environment (Jilin University), Ministry of Education, Jilin Provincial Key Laboratory of Water Resources and Environment, College of New Energy and Environment, Jilin University, Changchun 130021, China; College of Environmental Science and Engineering, Jilin Normal University, 1301 Haifeng Road, Siping 136000, China.
² Key Laboratory of Groundwater Resources and Environment (Jilin University), Ministry of Education, Jilin Provincial Key Laboratory of Water Resources and Environment, College of New Energy and Environment, Jilin University, Changchun 130021, China.
³ Key Laboratory of Urban Stormwater System and Water Environment, Ministry of Education, School of Environment and Energy Engineering, Beijing University of Civil Engineering and Architecture, Beijing 100044, China.
⁴ Key Laboratory of Groundwater Resources and Environment (Jilin University), Ministry of Education, Jilin Provincial Key Laboratory of Water Resources and Environment, College of New Energy and Environment, Jilin University, Changchun 130021, China; College of Environmental Science and Engineering, Jilin Normal University, 1301 Haifeng Road, Siping 136000, China; Key Laboratory of Urban Stormwater System and Water Environment, Ministry of Education, School of Environment and Energy Engineering, Beijing University of Civil Engineering and Architecture, Beijing 100044, China.
⁵ Key Laboratory of Groundwater Resources and Environment (Jilin University), Ministry of Education, Jilin Provincial Key Laboratory of Water Resources and Environment, College of New Energy and Environment, Jilin University, Changchun 130021, China. Electronic address: yxpiao@jlu.edu.cn.

PMID: 31000159
DOI: 10.1016/j.enzmictec.2019.03.006

Abstract

We developed a high efficiency bisphenol A (BPA) treating system in a fluidized bed reactor (FBR) using stabilized laccase (Lac) in mesoporous silica (SER/Lac). The SER/Lac, prepared by cross linking of each enzymes inside the porous silica using glutaraldehyde, presented improved stability than the free Lac over various pH and temperatures with shaking. When the SER/Lac was used for BPA biotransformation in a batch reaction, higher efficiency was achieved than the free or adsorbed Lac (ADS/Lac). Also, the SER/Lac presented better reusability compared to ADS/Lac, and it could be reused for three times without decrease of biotransformation efficiency, and half of it was remained after ten times use. Due to great stability and robustness property, SER/Lac was successfully applied for high efficiency and continuous BPA treatment in FBR with better performance than the batch reaction.

Keywords: Bisphenol A; Crosslinking; Fluidized bed reactor; Laccase; Mesoporous silica.

MeSH terms

Benzhydryl Compounds / chemistry*
Bioreactors*
Biotransformation*
Enzyme Stability
Enzymes, Immobilized / chemistry
Enzymes, Immobilized / metabolism*
Laccase / chemistry*
Laccase / metabolism*
Phenols / chemistry*
Porosity
Silicon Dioxide / chemistry*
Wastewater / chemistry

Substances

Benzhydryl Compounds
Enzymes, Immobilized
Phenols
Waste Water
Silicon Dioxide
Laccase
bisphenol A