Characterization of commercial green tea leaves by the analysis of low molecular weight carbohydrates and other quality indicators

Food Chem. 2019 Aug 30:290:159-167. doi: 10.1016/j.foodchem.2019.03.069. Epub 2019 Mar 15.

Abstract

A comprehensive characterization of commercial green tea (CGT) with the employment of independent variables such as low molecular weight carbohydrates (LMWC), soluble solids, color and antioxidant capacity has been performed in this manuscript. Within the manuscript, a convenient HILIC-MS method, suitable to perform a simultaneous identification and quantification of all mono-, di-, oligosaccharides and cyclitols observed in green tea was introduced. The method covers all key analytes in a single chromatographic analysis. Fifty-six samples from different origins (n = 10) were evaluated to explore differences based on origin. In addition, commercial samples processed by pan-firing and steaming were used for comparative purposes, allowing the identification of putative processing markers. The results obtained contribute to gain a better knowledge of the variations, according to origin and processing, in composition and quality of CGT, commodity widely appreciated by the consumers.

Keywords: Carbohydrates; Green tea; HILIC-MS; Multivariate statistical analysis; Quality parameters.

MeSH terms

  • Antioxidants / chemistry
  • Carbohydrates / analysis*
  • Chromatography, High Pressure Liquid
  • Disaccharides / analysis
  • Molecular Weight
  • Monosaccharides / analysis
  • Oligosaccharides / analysis
  • Plant Leaves / chemistry
  • Plant Leaves / metabolism
  • Principal Component Analysis
  • Spectrometry, Mass, Electrospray Ionization
  • Tea / chemistry*
  • Tea / metabolism

Substances

  • Antioxidants
  • Carbohydrates
  • Disaccharides
  • Monosaccharides
  • Oligosaccharides
  • Tea