Increase in the diagnosis of mycobacteremia after the implementation of semi-automated blood culture at a Brazilian public health laboratory

Rev Soc Bras Med Trop. 2019 Apr 11:52:e20180267. doi: 10.1590/0037-8682-0267-2018.

Abstract

Introduction: The prevalence of hematogenous dissemination of mycobacteria is high in immunosuppressed patients. The isolation of mycobacteria in culture remains the standard procedure.

Methods: This is a cross-sectional study based on the results of solid (Löwenstein-Jensen medium) and semi-automated liquid (BACTEC 9240) blood cultures, obtained from the Lacen-GO database.

Results: The implementation of a semi-automated procedure resulted in an increase of 61.5% and 350.0% in the positive results for Mycobacterium tuberculosis complex and nontuberculous mycobacteria, respectively. This technique also accelerated the detection of positive results.

Conclusions: Semi-automated liquid blood culture showed a better performance in the diagnosis of mycobacteremia.

MeSH terms

  • Automation, Laboratory / methods*
  • Bacteriological Techniques / methods*
  • Cross-Sectional Studies
  • Culture Media
  • Humans
  • Mycobacterium / isolation & purification*
  • Reagent Kits, Diagnostic
  • Sensitivity and Specificity
  • Time Factors

Substances

  • Culture Media
  • Reagent Kits, Diagnostic