Data on validation using accuracy profile of HPLC-UV method

Ahmed M Ibrahim; Hassan A M Hendawy; Wafaa S Hassan; Abdalla Shalaby; Heba M El-Sayed

doi:10.1016/j.dib.2019.103877

Data on validation using accuracy profile of HPLC-UV method

Data Brief. 2019 Mar 26:24:103877. doi: 10.1016/j.dib.2019.103877. eCollection 2019 Jun.

Authors

Ahmed M Ibrahim¹, Hassan A M Hendawy¹, Wafaa S Hassan², Abdalla Shalaby², Heba M El-Sayed²

Affiliations

¹ National Organization for Drug Control and Research, P.O. Box 29, Cairo, Egypt.
² Department of Analytical Chemistry, Faculty of Pharmacy, Zagazig University, Zagazig, Egypt.

Abstract

The data presented are related to the article entitled "Six Sigma quality approach for HPLC-UV method optimization" Ibrahim et al., 2019. The raw data of HPLC analysis of ascorbic acid (AS), paracetamol (PA) and guaifenesin (GU) are presented. Calibration standards were prepared at six concentrations levels (25%, 50%, 75%, 100%, 125% and 150%) each day and measured in triplicate. Validation standards were prepared at four concentration levels (25%, 60%, 100% and 150%) each day and measured in quintet. Three different series were used for method validation and prepared at the rate of one series per day.

Keywords: Accuracy profile; Ascorbic acid; Guaifenesin; HPLC; Paracetamol.