Biosynthesis of DHGA12 and its roles in Arabidopsis seedling establishment

Hao Liu; Siyi Guo; Minghua Lu; Yu Zhang; Junhua Li; Wei Wang; Pengtao Wang; Junli Zhang; Zhubing Hu; Liangliang Li; Lingyu Si; Jie Zhang; Qi Qi; Xiangning Jiang; José Ramón Botella; Hua Wang; Chun-Peng Song

doi:10.1038/s41467-019-09467-5

Biosynthesis of DHGA₁₂ and its roles in Arabidopsis seedling establishment

Nat Commun. 2019 Apr 16;10(1):1768. doi: 10.1038/s41467-019-09467-5.

Authors

Hao Liu^{1

2}, Siyi Guo^{1

2}, Minghua Lu³, Yu Zhang^{1

2}, Junhua Li⁴, Wei Wang^{1

2}, Pengtao Wang^{1

2}, Junli Zhang^{1

2}, Zhubing Hu^{1

2}, Liangliang Li^{1

2}, Lingyu Si^{1

2}, Jie Zhang^{1

2}, Qi Qi⁵, Xiangning Jiang⁵, José Ramón Botella^{1

2

6}, Hua Wang⁷, Chun-Peng Song^{8

9}

Affiliations

¹ Key Laboratory of Plant Stress Biology, School of Life Sciences, Henan University, 475004, Kaifeng, China.
² State Key Laboratory of Cotton Biology, School of Life Sciences, Henan University, 475004, Kaifeng, China.
³ College of Chemistry and Chemical Engineering, Henan University, 475004, Kaifeng, China.
⁴ College of Life Sciences, Henan Normal University, 453007, Xinxiang, China.
⁵ College of Biological Sciences and Biotechnology, Beijing Forestry University, 100083, Beijing, China.
⁶ Plant Genetic Engineering Laboratory, School of Agriculture and Food Sciences, The University of Queensland, Brisbane, QLD, 4072, Australia.
⁷ Engineering Research Center for Nanomaterials, Henan University, 475004, Kaifeng, China.
⁸ Key Laboratory of Plant Stress Biology, School of Life Sciences, Henan University, 475004, Kaifeng, China. songcp@henu.edu.cn.
⁹ State Key Laboratory of Cotton Biology, School of Life Sciences, Henan University, 475004, Kaifeng, China. songcp@henu.edu.cn.

Abstract

Seed germination and photoautotrophic establishment are controlled by the antagonistic activity of the phytohormones gibberellins (GAs) and abscisic acid (ABA). Here we show that Arabidopsis thaliana GAS2 (Gain of Function in ABA-modulated Seed Germination 2), a protein belonging to the Fe-dependent 2-oxoglutarate dioxygenase superfamily, catalyzes the stereospecific hydration of GA₁₂ to produce GA₁₂ 16, 17-dihydro-16α-ol (DHGA₁₂). We show that DHGA₁₂, a C₂₀-GA has an atypical structure compared to known active GAs but can bind to the GA receptor (GID1c). DHGA₁₂ can promote seed germination, hypocotyl elongation and cotyledon greening. Silencing and over-expression of GAS2 alters the ABA/GA ratio and sensitivity to ABA during seed germination and photoautotrophic establishment. Hence, we propose that GAS2 acts to modulate hormonal balance during early seedling development.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Abscisic Acid / metabolism
Arabidopsis / physiology*
Arabidopsis Proteins / genetics
Arabidopsis Proteins / metabolism*
Dioxygenases / genetics
Dioxygenases / metabolism*
Gibberellins / metabolism*
Loss of Function Mutation
Plants, Genetically Modified
Seedlings / growth & development*
Seeds / metabolism

Substances

Arabidopsis Proteins
Gibberellins
Abscisic Acid
At2g36690 protein, Arabidopsis
Dioxygenases