Mucin binding to therapeutic molecules: The case of antimicrobial agents used in cystic fibrosis

Cosmin Butnarasu; Nadia Barbero; Daniela Pacheco; Paola Petrini; Sonja Visentin

doi:10.1016/j.ijpharm.2019.04.032

Mucin binding to therapeutic molecules: The case of antimicrobial agents used in cystic fibrosis

Int J Pharm. 2019 Jun 10:564:136-144. doi: 10.1016/j.ijpharm.2019.04.032. Epub 2019 Apr 13.

Authors

Cosmin Butnarasu¹, Nadia Barbero², Daniela Pacheco³, Paola Petrini³, Sonja Visentin⁴

Affiliations

¹ Molecular Biotechnology and Health Science Department, University of Torino, Via Gioachino Quarello 15, 10135 Torino, Italy.
² NIS Interdepartmental and INSTM Reference Centre, Department of Chemistry, University of Torino, Via Pietro Giuria 7, 10125 Torino, Italy.
³ Department of Chemistry, Materials and Chemical Engineering "Giulio Natta", Politecnico di Milano, Piazza Leonardo da Vinci 32, 20133 Milan, Italy.
⁴ Molecular Biotechnology and Health Science Department, University of Torino, Via Gioachino Quarello 15, 10135 Torino, Italy. Electronic address: sonja.visentin@unito.it.

PMID: 30991132
DOI: 10.1016/j.ijpharm.2019.04.032

Abstract

Mucin is a complex glycoprotein consisting of a wide variety of functional groups that can interact with exogenous agents. The binding to mucin plays a crucial role in drug pharmacokinetics especially in diseases, such as cystic fibrosis (CF), where mucin is overexpressed. In this study, we have investigated the interaction between mucin and several drugs used in CF therapy. Protein-drug interaction was carried out by UV-Vis and fluorescence spectroscopy; quenching mechanism, binding constants, number of binding sites, thermodynamic parameters and binding distance of the interaction were obtained.

Keywords: Antibiotics; Cystic fibrosis; Fluorescence; Mucin; UV–Vis.

MeSH terms

Anti-Infective Agents / chemistry*
Binding Sites
Cystic Fibrosis / drug therapy
Mucins / chemistry*
Protein Binding

Substances

Anti-Infective Agents
Mucins