Two obligate anaerobic, Gram-stain-negative, non-spore-forming bacilli (strains SBC 8034T and SBC 8065) were isolated from brewery wastewater. Cells of the two strains were rod-shaped and 0.8×2-5 µm in size. Strains SBC 8034T and SBC 8065 did not grow on Columbia agar or tryptic soy agar II with 5 % sheep blood, brain-heart infusion agar or chocolate agar, but did grow on peptone-yeast-glucose agar and de Man, Rogosa and Sharpe agar using beer instead of water. The organisms produced acetic acid and succinic acid as the major metabolic end-products. Phylogenetic analysis based on 16S rRNA gene sequences revealed that both strains are clearly distinct from all recognized species within the genus Prevotella, but belong to the same species (DDH=85 %). Based on 16S rRNA and hsp60 gene sequencing, along with phenotypic, chemical and biochemical properties, strains SBC 8034T and SBC 8065 were considered to represent a novel species within the genus Prevotella, for which the name Prevotellacerevisiae sp. nov. is proposed. Strain SBC 8034T (=DSM 100619T=JCM 30867T) is the type strain of the proposed novel species.
Keywords: Bacteroidetes; Prevotella; beer spoilage.