Isolation of three distinct carbapenemase-producing Gram-negative bacteria from a Vietnamese medical tourist

Natsue Hosono Honda; Kotaro Aoki; Toshiro Kamisasanuki; Naoto Matsuda; Masako To; Hisao Matsushima; Yoshikazu Ishii; Kosuke Haruki

doi:10.1016/j.jiac.2019.03.020

Isolation of three distinct carbapenemase-producing Gram-negative bacteria from a Vietnamese medical tourist

J Infect Chemother. 2019 Oct;25(10):811-815. doi: 10.1016/j.jiac.2019.03.020. Epub 2019 Apr 12.

Authors

Natsue Hosono Honda¹, Kotaro Aoki², Toshiro Kamisasanuki³, Naoto Matsuda⁴, Masako To⁵, Hisao Matsushima³, Yoshikazu Ishii², Kosuke Haruki⁴

Affiliations

¹ Department of Laboratory Medicine, Dokkyo Medical University, Saitama Medical Center, 2-1-50 Minami-Koshigaya, Koshigaya City, Saitama 343-8555, Japan; Department of Infection Control & Infectious Diseases, Dokkyo Medical University, Saitama Medical Center, 2-1-50 Minami-Koshigaya, Koshigaya City, Saitama 343-8555, Japan. Electronic address: nhonda@dokkyomed.ac.jp.
² Department of Microbiology and Infectious Diseases, Faculty of Medicine, Toho University, Tokyo 143-8540, Japan.
³ Emergency and Critical Care Medicine, Dokkyo Medical University, Saitama Medical Center, 2-1-50 Minami-Koshigaya, Koshigaya City, Saitama 343-8555, Japan.
⁴ Department of Laboratory Medicine, Dokkyo Medical University, Saitama Medical Center, 2-1-50 Minami-Koshigaya, Koshigaya City, Saitama 343-8555, Japan; Department of Infection Control & Infectious Diseases, Dokkyo Medical University, Saitama Medical Center, 2-1-50 Minami-Koshigaya, Koshigaya City, Saitama 343-8555, Japan.
⁵ Department of Laboratory Medicine, Dokkyo Medical University, Saitama Medical Center, 2-1-50 Minami-Koshigaya, Koshigaya City, Saitama 343-8555, Japan.

PMID: 30987949
DOI: 10.1016/j.jiac.2019.03.020

Abstract

Carbapenem-resistant Klebsiella pneumoniae and Escherichia coli, multidrug-resistant Pseudomonas aeruginosa and vancomycin-resistant Enterococcus faecium were isolated from a single patient. The patient came to Japan for advanced medical treatment after having undergone laparoscopic cholecystectomy and hospitalization in Vietnam. Whole-genome sequence analysis revealed that K. pneumoniae harbored bla_OXA-48 that was found on a Col156 -type small plasmid, E. coli harbored bla_NDM-5 and P. aeruginosa harbored both bla_NDM-1 and 16S rRNA methyltransferase (rmtB). To the best of our knowledge, this is the first report of detection of K. pneumoniae harboring bla_OXA-48 on a Col156-type small plasmid in the world and P. aeruginosa coharboring genes encoding NDM-1 and RmtB in Japan.

Keywords: Col156; Medical tourism; NDM-1; NDM-5; OXA-48; RmtB.

Publication types

Case Reports

MeSH terms

Bacterial Proteins / genetics*
Bacterial Proteins / metabolism
Carbapenem-Resistant Enterobacteriaceae / drug effects
Carbapenem-Resistant Enterobacteriaceae / genetics
Carbapenem-Resistant Enterobacteriaceae / isolation & purification
Drug Resistance, Multiple, Bacterial / genetics
Escherichia coli / drug effects
Escherichia coli / genetics
Escherichia coli / isolation & purification
Gram-Negative Bacterial Infections / diagnosis
Gram-Negative Bacterial Infections / drug therapy
Gram-Negative Bacterial Infections / microbiology*
Humans
Japan
Klebsiella pneumoniae / drug effects
Klebsiella pneumoniae / genetics
Klebsiella pneumoniae / isolation & purification
Male
Medical Tourism
Middle Aged
Plasmids / genetics
Plasmids / metabolism
Pseudomonas aeruginosa / drug effects
Pseudomonas aeruginosa / genetics
Pseudomonas aeruginosa / isolation & purification
Vancomycin-Resistant Enterococci / drug effects
Vancomycin-Resistant Enterococci / genetics
Vancomycin-Resistant Enterococci / isolation & purification
Vietnam
beta-Lactam Resistance / genetics
beta-Lactamases / genetics*
beta-Lactamases / metabolism

Substances

Bacterial Proteins
beta-Lactamases
carbapenemase