Detection of BPDE-DNA adducts in human umbilical cord blood by LC-MS/MS analysis

J Food Drug Anal. 2019 Apr;27(2):518-525. doi: 10.1016/j.jfda.2019.03.001. Epub 2019 Mar 23.

Abstract

Benzo [a]pyrene (BaP) is a model compound for the study of polycyclic aromatic hydrocarbon (PAH) carcinogenesis. Upon metabolism, BaP is metabolized to the ultimate metabolite, BaP trans-7,8-diol-anti-9,10-epoxide (BPDE), that reacts with cellular DNA to form BPDE-dG adducts responsible for BaP-induced mutagenicity, carcinogenicity, and teratogenicity. In this study, we employed our developed LC-MS/MS method to detect and quantity BPDE-dG adducts present in 42 normal human umbilical cord blood samples and 42 birth defect cases. We determined that there is no significant difference in the level of BPDE-dG formation between the normal and birth defect groups. This represents the first time to use an LC-MS/MS method to quantify BPDE-dG in human umbilical blood samples. The results indicated that under experimental conditions, BPDE-dG adducts were detected in all the human umbilical cord blood samples from the normal and birth defect groups.

Keywords: BPDE-dG; Human biomonitoring; LC-MS/MS; Molecular epidemiology.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide / chemistry
  • Chromatography, Liquid
  • DNA Adducts / blood*
  • DNA Adducts / chemistry
  • Female
  • Fetal Blood / chemistry*
  • Humans
  • Molecular Conformation
  • Pregnancy
  • Tandem Mass Spectrometry

Substances

  • DNA Adducts
  • benzo(a)pyrene-7,8-dihydrodiol-9,10-epoxide-DNA
  • 7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide

Grants and funding

The research work was supported by National Science and Technology Infrastructure Program, China (2013BAI12B03).