Abstract
A DSN-RNAse-TdT-T7 exo probing system allows the detection of miRNA 21 with very high sensitivity (LOD = 2.57 fM) and selectivity-the result of (i) avoiding the false-positive signal from miRNA reacting with TdT polymerase and (ii) signal amplification occurring through a FRET-breaking mechanism involving T7 exo.
MeSH terms
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Bacteriophage T7 / enzymology
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DNA Nucleotidylexotransferase / chemistry*
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DNA Probes / chemical synthesis
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DNA Probes / genetics
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DNA, Single-Stranded / chemical synthesis
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DNA, Single-Stranded / genetics
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Exodeoxyribonucleases / chemistry*
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Fluorescence Resonance Energy Transfer / methods
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Fluorescent Dyes / chemistry
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Humans
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Limit of Detection
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MicroRNAs / blood*
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MicroRNAs / genetics
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Nucleic Acid Amplification Techniques
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Nucleic Acid Hybridization
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RNA, Messenger / genetics
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Ribonucleases / chemistry*
Substances
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DNA Probes
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DNA, Single-Stranded
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Fluorescent Dyes
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MIRN21 microRNA, human
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MicroRNAs
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RNA, Messenger
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DNA Nucleotidylexotransferase
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Exodeoxyribonucleases
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Ribonucleases
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gene b exonuclease