An antibody-based immunotherapy for methamphetamine (MA) addictive treatment is has been drawing more and more attention in recent years. However, studies about methamphetamine antibody (anti-MA) immunodetections are rare, owing to the lack of immunogenicity of small molecule MA. This study provides a simple and effective approach to develop a convenient electrochemiluminescent (ECL) immunosensor for the testing of anti-MA. In short, the synthetic holoantigen of MA is immobilized on a homemade gold nanoparticles modified electrode as the sensing host for the specific recognition and detection of anti-MA. The research suggested, under optimal experimental conditions, the ECL intensity on resultant immunosensor has a wide-linear regression toward the anti-MA quantity within the range from 0.03 to 3.07 ng with a detection limit of 2.32 pg. It responded to the dosage of anti-MA in spiked blood samples with satisfactory recovery. According to the research, the developed sensor shows promise as a portable Anti-MA fast seized device which performs quickly and offers convenience, and will be helpful for forensic identification and clinical treatment.
Keywords: Electrochemiluminescence; drug addiction therapy; immunosensor; methamphetamine; methamphetamine antibody.