Liquefaction of chicken sternal cartilage by steam explosion to isolate chondroitin sulfate

Qingshan Shen; Chunhui Zhang; Wei Jia; Xiaojie Qin; Xiong Xu; Mengliang Ye; Haizhen Mo; Aurore Richel

doi:10.1016/j.carbpol.2019.03.032

Liquefaction of chicken sternal cartilage by steam explosion to isolate chondroitin sulfate

Carbohydr Polym. 2019 Jul 1:215:73-81. doi: 10.1016/j.carbpol.2019.03.032. Epub 2019 Mar 15.

Authors

Qingshan Shen¹, Chunhui Zhang², Wei Jia³, Xiaojie Qin³, Xiong Xu³, Mengliang Ye³, Haizhen Mo⁴, Aurore Richel⁵

Affiliations

¹ Key Laboratory of Agro-Products Processing, Ministry of Agriculture and Rural Affairs, Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing, 100193, China; University of Liege-Gembloux Agro-Bio Tech, Laboratory of Biomass and Green Technologies, Passage des déportés 2, B-5030, Gembloux, Belgium.
² Key Laboratory of Agro-Products Processing, Ministry of Agriculture and Rural Affairs, Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing, 100193, China. Electronic address: dr_zch@163.com.
³ Key Laboratory of Agro-Products Processing, Ministry of Agriculture and Rural Affairs, Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.
⁴ Department of Food Science, Henan Institute of Science and Technology, Xinxiang, 453003, China.
⁵ University of Liege-Gembloux Agro-Bio Tech, Laboratory of Biomass and Green Technologies, Passage des déportés 2, B-5030, Gembloux, Belgium.

PMID: 30981372
DOI: 10.1016/j.carbpol.2019.03.032

Abstract

Chondroitin sulfate (CS), together with peptide, was isolated from the liquid fraction of chicken sternal cartilage subjected to steam explosion (SE) by membrane separation. Cartilage was liquefied via the SE conditions, including various pressures (1.0-1.6 MPa) and times (60-140 s). The extraction procedure was optimized as follows: the amount of papain added, 0.11%; enzymolysis time, 10.5 h; and enzymolysis temperature, 56.5 °C, under which the highest recovery and total yield of CS were 92.15% and 18.55% at 1.4 MPa for120 s, and the counterparts of peptides were 87.35% (1.0 MPa, 140 s) and 63.07% (1.6 MPa, 140 s). The average molecular weight of CS samples ranged from 30 to 35 kDa. CS sample was confirmed using agarose-gel electrophoresis, and the structure was analysed Fourier transform infrared spectroscopy, chromatography and nuclear magnetic resonance. Taken together, SE can be an eco-friendly pretreatment method to liquefy cartilage for CS isolation.

Keywords: Cartilage; Chondroitin sulfate; Liquefaction; Steam explosion.

MeSH terms

Animals
Chemical Fractionation / methods*
Chickens
Chondroitin Sulfates / isolation & purification*
Costal Cartilage / chemistry*
Phase Transition*
Steam*

Substances

Steam
Chondroitin Sulfates