Quantitative Phosphoproteomic Using Titanium Dioxide Micro-Columns and Label-Free Quantitation

Martin E Barrios-Llerena; Thierry Le Bihan

doi:10.1007/978-1-4939-9232-4_4

Quantitative Phosphoproteomic Using Titanium Dioxide Micro-Columns and Label-Free Quantitation

Methods Mol Biol. 2019:1977:35-42. doi: 10.1007/978-1-4939-9232-4_4.

Authors

Martin E Barrios-Llerena¹, Thierry Le Bihan²

Affiliations

¹ Proteomics and Mass Spectrometry, Bioscience Core Labs, King Abdullah University of Science and Technology, Thuwal, Saudi Arabia. martin.barriosllerena@kaust.edu.sa.
² Synthetic and Systems Biology, School of Biological Sciences, University of Edinburgh, Edinburgh, UK.

PMID: 30980321
DOI: 10.1007/978-1-4939-9232-4_4

Abstract

Phosphorylation events are important during cellular function. Analysis of phosphorylation in complex samples has been extensively studied using large-scale phosphopeptide enrichment methods. Quantitative analysis of the enriched phosphopeptides is subsequently performed using label-based methodologies (e.g., SILAC, iTRAQ, and others). Here we describe the protocol for the quantitative analysis of phosphopeptides, enriched with titanium dioxide micro-column, using an intensity-based label-free quantitation.

Keywords: Label-free quantitation; Mass spectrometry; Phosphopeptide enrichment; Titanium dioxide chromatography (TiO2).

MeSH terms

Chromatography, Affinity* / methods
Data Analysis
Phosphopeptides / chemistry
Phosphopeptides / metabolism
Phosphoproteins / chemistry
Phosphoproteins / metabolism*
Phosphorylation
Proteomics / methods*
Titanium* / chemistry

Substances

Phosphopeptides
Phosphoproteins
titanium dioxide
Titanium