A fully portable microchip real-time polymerase chain reaction for rapid detection of pathogen

Electrophoresis. 2019 Jul;40(12-13):1699-1707. doi: 10.1002/elps.201900090. Epub 2019 Apr 25.

Abstract

Point-of-care detection for pathogen is of critical need for wide epidemic warning and medical diagnosis. In this work, we have designed and developed a fully portable and integrated microchip based real-time polymerase chain reaction machine for rapid pathogen detection. The instrument consists of three functional components including heating, optical, and electrical modules, which are integrated into a portable compact box. The microchip is consumable material replaceable to meet various detection needs. Consequently, we demonstrated the outstanding performance of this portable machine for rapid detection of Salmonella and Escherichia coli O157:H7 with the advantage of time-saving (∼25 min), less samples consumption, portability, and user-friendly operation.

Keywords: DNA; Microchip real-time PCR; Pathogen; Point-of-care testing.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • DNA, Bacterial* / analysis
  • DNA, Bacterial* / genetics
  • DNA, Bacterial* / isolation & purification
  • Equipment Design
  • Escherichia coli O157 / genetics
  • Escherichia coli O157 / isolation & purification
  • HeLa Cells
  • Humans
  • Molecular Typing* / instrumentation
  • Molecular Typing* / methods
  • Point-of-Care Testing*
  • Real-Time Polymerase Chain Reaction* / instrumentation
  • Real-Time Polymerase Chain Reaction* / methods
  • Salmonella / genetics
  • Salmonella / isolation & purification

Substances

  • DNA, Bacterial