Effects of manganese incorporation on the morphology, structure and cytotoxicity of spherical bioactive glass nanoparticles

Breno R Barrioni; Parichart Naruphontjirakul; Elizabeth Norris; Siwei Li; Nicole L Kelly; John V Hanna; Molly M Stevens; Julian R Jones; Marivalda de M Pereira

doi:10.1016/j.jcis.2019.04.016

Effects of manganese incorporation on the morphology, structure and cytotoxicity of spherical bioactive glass nanoparticles

J Colloid Interface Sci. 2019 Jul 1:547:382-392. doi: 10.1016/j.jcis.2019.04.016. Epub 2019 Apr 4.

Authors

Breno R Barrioni¹, Parichart Naruphontjirakul², Elizabeth Norris³, Siwei Li⁴, Nicole L Kelly⁵, John V Hanna⁶, Molly M Stevens⁷, Julian R Jones⁸, Marivalda de M Pereira⁹

Affiliations

¹ Department of Metallurgical Engineering and Materials, Federal University of Minas Gerais, School of Engineering, Belo Horizonte, MG, Brazil. Electronic address: brenorb@ufmg.br.
² Biological Engineering Program, King Mongkut's University of Technology Thonburi, Thailand; Department of Materials, Imperial College London, South Kensington, London SW7 2AZ, UK. Electronic address: parichart.nar@kmutt.ac.th.
³ Department of Materials, Imperial College London, South Kensington, London SW7 2AZ, UK. Electronic address: e.norris14@imperial.ac.uk.
⁴ Department of Materials, Imperial College London, South Kensington, London SW7 2AZ, UK. Electronic address: siwei.li@imperial.ac.uk.
⁵ MAS CDT, Senate House, University of Warwick, Coventry CV4 7AL, UK; Department of Physics, University of Warwick, Coventry CV4 7AL, UK. Electronic address: n.kelly.1@warwick.ac.uk.
⁶ Department of Physics, University of Warwick, Coventry CV4 7AL, UK. Electronic address: j.v.hanna@warwick.ac.uk.
⁷ Department of Materials, Imperial College London, South Kensington, London SW7 2AZ, UK. Electronic address: m.stevens@imperial.ac.uk.
⁸ Department of Materials, Imperial College London, South Kensington, London SW7 2AZ, UK. Electronic address: julian.r.jones@imperial.ac.uk.
⁹ Department of Metallurgical Engineering and Materials, Federal University of Minas Gerais, School of Engineering, Belo Horizonte, MG, Brazil. Electronic address: mpereira@demet.ufmg.br.

PMID: 30974253
DOI: 10.1016/j.jcis.2019.04.016

Abstract

Bioactive glass nanoparticles (BGNPs) are of great interest in tissue engineering as they possess high dissolution rate and capability of being internalized by cells, releasing their dissolution products with therapeutic benefits intracellularly. A modified Stöber process can be applied to obtain different BGNPs compositions containing therapeutic ions while maintaining controllable particle morphology, monodispersity and reduce agglomeration. Here, BGNPs containing Mn, an ion that has been shown to influence the osteoblast proliferation and bone mineralization, were evaluated. Particles with up to 142.3 ± 10.8 nm and spherical morphology were obtained after MnO incorporation in the SiO₂ - CaO system. X-ray photoelectron spectroscopy (XPS) indicated the presence of Mn²⁺ species and also a reduction in the number of bridging oxygen bonds due to the Ca and Mn. The Ca and Mn network modifier role on the silica network was also confirmed by magic-angle spinning ²⁹Si solid-state nuclear magnetic resonance (MAS NMR). MTT evaluation showed no reduction in the mitochondrial metabolic activity of human mesenchymal stem cells exposed to the glass ionic products. Thus, evaluation showed that Mn could be incorporated into BGNPs by the modified Stöber method while maintaining their spherical morphology and features as a promising strategy for tissue regeneration.

Keywords: Bioactive glass; Manganese; Nanoparticles; Stöber.

MeSH terms

Cell Survival / drug effects
Cells, Cultured
Glass / chemistry*
Humans
Manganese / chemistry
Manganese / pharmacology*
Mesenchymal Stem Cells / drug effects*
Nanoparticles / chemistry*
Particle Size
Surface Properties

Substances

Manganese