Biological variation of serum canine calprotectin concentrations as measured by ELISA in healthy dogs

R M Heilmann; C G Ruaux; N Grützner; S M Cranford; C S Bridges; J M Steiner

doi:10.1016/j.tvjl.2019.03.002

Biological variation of serum canine calprotectin concentrations as measured by ELISA in healthy dogs

Vet J. 2019 May:247:61-64. doi: 10.1016/j.tvjl.2019.03.002. Epub 2019 Mar 16.

Authors

R M Heilmann¹, C G Ruaux², N Grützner³, S M Cranford⁴, C S Bridges⁴, J M Steiner⁴

Affiliations

¹ Department for Small Animals, Veterinary Teaching Hospital, College of Veterinary Medicine, University of Leipzig, An den Tierkliniken 23, DE-04103 Leipzig, SN, Germany; Gastrointestinal Laboratory, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, 4474 TAMU, College Station, TX 77843, USA. Electronic address: romy.heilmann@kleintierklinik.uni-leipzig.de.
² Veterinary Teaching Hospital, School of Veterinary Medicine, Massey University, Private Bag 11 222, Palmerston North 4442, New Zealand.
³ Gastrointestinal Laboratory, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, 4474 TAMU, College Station, TX 77843, USA; Institute of Agricultural and Nutritional Sciences, Martin Luther University Halle-Wittenberg, Theodor-Lieser St 11, DE-06120 Halle (Saale), SA, Germany.
⁴ Gastrointestinal Laboratory, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, 4474 TAMU, College Station, TX 77843, USA.

PMID: 30971353
DOI: 10.1016/j.tvjl.2019.03.002

Abstract

Calprotectin is a useful biomarker of inflammation in dogs. However, the biological variation of serum canine calprotectin is unknown. Indices of biological variation were determined in serial serum samples (n=147) from 11 healthy dogs (males/females: 4/7, median age: 5 years): analytical (3.0%), intra-individual (29.9%), and inter-individual variation (33.2%), reciprocal index of individuality (1.1), and index of heterogeneity (4.9). Serum calprotectin concentrations measured by ELISA and by the previous radioimmunoassay were highly correlated, but a constant and proportional bias exists between both assays. A de novo ELISA-reference interval (RI) for serum calprotectin concentration was established (0.6-11.8mg/L). Moderate changes in serum calprotectin (minimum critical difference: 6.4mg/L) between sequential measurements are needed to be considered relevant, and a population-based RI may or may not be appropriate for serum calprotectin.

Keywords: Calgranulin A; Calgranulin B; Dog; Reference change value; S100A8/A9.

MeSH terms

Animals
Biological Variation, Individual
Dogs / blood*
Dogs / immunology
Enzyme-Linked Immunosorbent Assay / veterinary*
Female
Inflammation Mediators / blood
Leukocyte L1 Antigen Complex / blood*
Male

Substances

Inflammation Mediators
Leukocyte L1 Antigen Complex