The aim of this study was to fabricate biodegradable poly-l-lactic acid (PLLA) bone screws containing iron oxide (Fe₃O₄) nanoparticles, which are radiopaque and 3D-printable. The PLLA composites were fabricated by loading 20%, 30%, and 40% Fe₃O₄ nanoparticles into the PLLA. The physical properties, including elastic modulus, thermal properties, and biocompatibility of the composites were tested. The 20% nano-Fe₃O₄/PLLA composite was used as the material for fabricating the 3D-printed bone screws. The mechanical performance of the nano-Fe₃O₄/PLLA bone screws was evaluated by anti-bending and anti-torque strength tests. The tissue response and radiopacity of the nano-Fe₃O₄/PLLA bone screws were assessed by histologic and CT imaging studies using an animal model. The addition of nano-Fe₃O₄ increased the crystallization of the PLLA composites. Furthermore, the 20% nano-Fe₃O₄/PLLA composite exhibited the highest thermal stability compared to the other Fe₃O₄ proportions. The 3D-printed bone screws using the 20% nano-Fe₃O₄/PLLA composite provided excellent local tissue response. In addition, the radiopacity of the 20% nano-Fe₃O₄/PLLA screw was significantly better compared with the neat PLLA screw.
Keywords: 3D printed bone screw; iron oxide nanoparticles; poly-l-lactic acid; radiopaque polymer.