Background: One of the major problems in wound healing is scar formation; however, there are few ways to prevent or treat it. Different species of Achillea are used to treat wounds in folk medicine from the past but there are few studies on the effect of it on wound healing and inhibition of scar formation. The aim of this study was to investigate the effect of Achillea biebersteinii Afan hydroethanolic extract on the expression of TGFβ1 and bFGF as effective growth factors of wound healing in mouse embryonic fibroblast cells.
Methods: Mouse embryonic fibroblast cells were exposed to different concentrations of Achillea extract at two different time (12 and 24 hr); the expression of TGFβ1 and bFGF was performed by real-time-PCR and ELISA at the level of gene and protein.
Results: It was observed that the plant extract at 5 and 10 µg/ml downregulated the expression of TGFβ1 and upregulated the expression of bFGF at the level of gene and protein.
Conclusion: The results showed that the pattern of changes in the expression of TGFβ1 and bFGF by Achillea biebersteinni Afan extract may inhibit scar formation.
Keywords: Achillea biebersteinni Afan; TGFβ1; bFGF; fibroblast; scar formation.
© 2019 The Authors. Molecular Genetics & Genomic Medicine published by Wiley Periodicals, Inc.