Quantitation of mRNA Transcripts and Proteins Using the BD Rhapsody™ Single-Cell Analysis System

Eleen Y Shum; Elisabeth M Walczak; Christina Chang; H Christina Fan

doi:10.1007/978-981-13-6037-4_5

Quantitation of mRNA Transcripts and Proteins Using the BD Rhapsody™ Single-Cell Analysis System

Adv Exp Med Biol. 2019:1129:63-79. doi: 10.1007/978-981-13-6037-4_5.

Authors

Eleen Y Shum¹, Elisabeth M Walczak², Christina Chang², H Christina Fan²

Affiliations

¹ BD Biosciences, San Jose, CA, USA. Eleen.Shum@bd.com.
² BD Biosciences, San Jose, CA, USA.

PMID: 30968361
DOI: 10.1007/978-981-13-6037-4_5

Abstract

In this review, we describe the BD Rhapsody™ Single-Cell Analysis System, a platform that allows high-throughput capture of nucleic acids from single cells using a simple cartridge workflow and a multitier barcoding system. The resulting captured information can be used to generate various types of next-generation sequencing (NGS) libraries, including whole transcriptome analysis for discovery biology and targeted RNA analysis for high sensitivity transcript detection. The BD Rhapsody system can be used with emerging applications, such as BD™ AbSeq assays, to profile gene expression in both mRNA and protein level to provide ultra-high resolution analysis of single cells.

Keywords: Antibody; Barcoded beads; Magnetic beads; Microwell array; Molecular barcoding; Next-generation sequencing; Single cells; Targeted RNA-seq; Transcriptome; UMI; Unique molecular identifiers.

Publication types

Review

MeSH terms

Gene Expression Profiling*
High-Throughput Nucleotide Sequencing*
RNA, Messenger / analysis*
Sequence Analysis, RNA
Single-Cell Analysis / instrumentation*
Single-Cell Analysis / methods*
Transcriptome

Substances

RNA, Messenger