Effects of NH4+-N and NO2--N on carbon fixation in an anaerobic ammonium oxidation reactor

Huaqin Wang; Jun Han; Wenjie Zhang

doi:10.1016/j.jenvman.2019.02.034

Effects of NH₄⁺-N and NO₂^--N on carbon fixation in an anaerobic ammonium oxidation reactor

J Environ Manage. 2019 Jul 1:241:450-457. doi: 10.1016/j.jenvman.2019.02.034. Epub 2019 Apr 6.

Authors

Huaqin Wang¹, Jun Han², Wenjie Zhang³

Affiliations

¹ School of Resource and Environmental Engineering, Wuhan University of Science and Technology, Wuhan, 430081, China; Guangxi Key Laboratory of Environmental Pollution Control Theory and Technology, Guilin University of Technology, Guilin, 541004, China.
² School of Resource and Environmental Engineering, Wuhan University of Science and Technology, Wuhan, 430081, China.
³ Guangxi Key Laboratory of Environmental Pollution Control Theory and Technology, Guilin University of Technology, Guilin, 541004, China. Electronic address: 2010053@glut.edu.cn.

PMID: 30967351
DOI: 10.1016/j.jenvman.2019.02.034

Abstract

Inorganic carbon (IC) is used as a carbon source of the anaerobic ammonium oxidation (anammox) microorganisms during the anammox process. Meanwhile, anammox microorganisms possess a certain carbon fixation capacity. In this study, the effects of NH₄⁺-N and NO₂^--N on carbon fixation in an anammox reactor were investigated. The carbon fixation content had a positive correlation with the amount of NH₄⁺-N and NO₂^--N. A high carbon sequestration of 6.52 mg-C and relatively low CO₂ emissions of 1.00 mg-C were caused by a high amount of influent nitrogen. The microbiology analysis showed that there was a significant relevance between the abundance of the cbbLR1 gene and the carbon fixation content. The results revealed that the Calvin cycle pathway for carbon fixation was used by the anammox bacteria, which may be uncultured Bacillus sp. clone TA_17 or uncultured Methylobacterium sp. clone LA8_13.

Keywords: Anammox; Carbon fixation; NH(4)(+)-N; NO(2)(−)-N; cbbLR1.

MeSH terms

Ammonium Compounds*
Anaerobiosis
Bioreactors*
Carbon Cycle
Nitrogen
Oxidation-Reduction

Substances

Ammonium Compounds
Nitrogen