Microencapsulation of Phosphorylated Human-Like Collagen-Calcium Chelates for Controlled Delivery and Improved Bioavailability

Yu Mi; Zhengfang Liu; Jianjun Deng; Huan Lei; Chenhui Zhu; Daidi Fan; Xingqiang Lv

doi:10.3390/polym10020185

Microencapsulation of Phosphorylated Human-Like Collagen-Calcium Chelates for Controlled Delivery and Improved Bioavailability

Polymers (Basel). 2018 Feb 14;10(2):185. doi: 10.3390/polym10020185.

Authors

Yu Mi^{1

2}, Zhengfang Liu^{3

4}, Jianjun Deng^{5

6}, Huan Lei^{7

8}, Chenhui Zhu^{9

10}, Daidi Fan^{11

12}, Xingqiang Lv^{13

14}

Affiliations

¹ Shaanxi Key Laboratory of Degradable Biomedical Materials, School of Chemical Engineering, Northwest University, 229 North Taibai Road, Xi'an 710069, Shaanxi, China. mi_yu@nwu.edu.cn.
² Shaanxi R&D Center of Biomaterials and Fermentation Engineering, School of Chemical Engineering, Northwest University, 229 North Taibai Road, Xi'an 710069, Shaanxi, China. mi_yu@nwu.edu.cn.
³ Shaanxi Key Laboratory of Degradable Biomedical Materials, School of Chemical Engineering, Northwest University, 229 North Taibai Road, Xi'an 710069, Shaanxi, China. zhengfangliu89@163.com.
⁴ Shaanxi R&D Center of Biomaterials and Fermentation Engineering, School of Chemical Engineering, Northwest University, 229 North Taibai Road, Xi'an 710069, Shaanxi, China. zhengfangliu89@163.com.
⁵ Shaanxi Key Laboratory of Degradable Biomedical Materials, School of Chemical Engineering, Northwest University, 229 North Taibai Road, Xi'an 710069, Shaanxi, China. dengjianjun@nwu.edu.cn.
⁶ Shaanxi R&D Center of Biomaterials and Fermentation Engineering, School of Chemical Engineering, Northwest University, 229 North Taibai Road, Xi'an 710069, Shaanxi, China. dengjianjun@nwu.edu.cn.
⁷ Shaanxi Key Laboratory of Degradable Biomedical Materials, School of Chemical Engineering, Northwest University, 229 North Taibai Road, Xi'an 710069, Shaanxi, China. 201520729@stumail.nwu.edu.cn.
⁸ Shaanxi R&D Center of Biomaterials and Fermentation Engineering, School of Chemical Engineering, Northwest University, 229 North Taibai Road, Xi'an 710069, Shaanxi, China. 201520729@stumail.nwu.edu.cn.
⁹ Shaanxi Key Laboratory of Degradable Biomedical Materials, School of Chemical Engineering, Northwest University, 229 North Taibai Road, Xi'an 710069, Shaanxi, China. zch2005@nwu.edu.cn.
¹⁰ Shaanxi R&D Center of Biomaterials and Fermentation Engineering, School of Chemical Engineering, Northwest University, 229 North Taibai Road, Xi'an 710069, Shaanxi, China. zch2005@nwu.edu.cn.
¹¹ Shaanxi Key Laboratory of Degradable Biomedical Materials, School of Chemical Engineering, Northwest University, 229 North Taibai Road, Xi'an 710069, Shaanxi, China. fandaidi@nwu.edu.cn.
¹² Shaanxi R&D Center of Biomaterials and Fermentation Engineering, School of Chemical Engineering, Northwest University, 229 North Taibai Road, Xi'an 710069, Shaanxi, China. fandaidi@nwu.edu.cn.
¹³ Shaanxi Key Laboratory of Degradable Biomedical Materials, School of Chemical Engineering, Northwest University, 229 North Taibai Road, Xi'an 710069, Shaanxi, China. lvxq@nwu.edu.cn.
¹⁴ Shaanxi R&D Center of Biomaterials and Fermentation Engineering, School of Chemical Engineering, Northwest University, 229 North Taibai Road, Xi'an 710069, Shaanxi, China. lvxq@nwu.edu.cn.

Abstract

The bioavailability of Phosphorylated Human-like Collagen-calcium chelates (PHLC-Ca) as calcium supplement is influenced by the extremely low pH and proteolytic enzymes in the gastrointestinal tract. This study addresses these issues by microencapsulation technology using alginate (ALG) and chitosan (CS) as wall materials. The different ratio of ALG to PHLC-Ca on microcapsules encapsulation efficiency (EE) and loading capacity (LC) was evaluated and 1:1/2 was selected as the optimal proportion. The microcapsules were micron-sized and spherical in shape. PHLC-Ca was successfully entrapped into the matrix of ALG through forming intermolecular hydrogen bonding or other interactions. The confocal laser scanning microscopy (CLSM) indicated that CS was coated on ALG microspheres. The MTT assay exhibited that CS/ALG-(PHLC-Ca) microcapsules extracts were safe to L929. The animal experiment showed that CS/ALG-(PHLC-Ca) microcapsules was superior to treating osteoporosis than PHLC-Ca. These results illustrated that the bioavailability of PHLC-Ca was improved by microencapsulated.

Keywords: PHLC-Ca; alginate; bioavailability; chitosan; microcapsules.

Abstract

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