The plant-based biological molecules possess exceptionally controlled assembling properties to make them suitable in the synthesis of metal nanoparticles. In the present study, an efficient simple one-pot method was employed for the synthesis of silver nanoparticles (SNPs) from the Rangoon creeper (RC) aqueous leaf extract. Biomolecules present in the leaf extract play a significant role as reducing agent as well as capping agent in the formation of RC-SNPs. The formation of RC-SNPs was confirmed by using several analytical techniques such as Fourier-transform infrared spectroscopy and ultraviolet-visible spectrophotometer studies. The presence of a sharp surface plasmon resonance peak at 449 nm showed the formation of RC-SNPs. X-ray diffraction analysis showed the crystalline nature of the RC-SNPs with a face-centred cubic structure. Elemental analysis of RC-SNPs was done by using energy-dispersive X-ray spectroscopy and X-ray photoelectron spectroscopy. The morphology of RC-SNPs was examined by transmission electron microscopy (TEM) in the nano range 12 nm, and thermogravimetric-differential thermal analysis demonstrated the mechanical strength of RC-SNPs at various temperatures. The authors' newly synthesised RC-SNPs exhibited significant anti-bacterial activity against Staphylococcus aureus and Escherichia coli.