Efficient gene disruption by posttransformational directed internal homologous recombination in Pichia pastoris

Liangcheng Jiao; Qinghua Zhou; Yunjun Yan

doi:10.1016/j.ab.2019.04.004

Efficient gene disruption by posttransformational directed internal homologous recombination in Pichia pastoris

Anal Biochem. 2019 Jul 1:576:1-4. doi: 10.1016/j.ab.2019.04.004. Epub 2019 Apr 6.

Authors

Liangcheng Jiao¹, Qinghua Zhou¹, Yunjun Yan²

Affiliations

¹ Key Laboratory of Molecular Biophysics of the Ministry of Education, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, PR China.
² Key Laboratory of Molecular Biophysics of the Ministry of Education, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, PR China. Electronic address: yanyunjun@hust.edu.cn.

PMID: 30958998
DOI: 10.1016/j.ab.2019.04.004

Abstract

In this study, we designed an improved method to construct gene-deficient strain in non-conventional yeast Pichia pastoris. This method achieved high efficiency of gene deletion without disruption of NHEJ-related genes. The disruption efficiency of Och1 reached about 80%. This simple method will be a new useful molecular tool for P. pastoris.

Keywords: Gene disruption; Homologous recombination; Non-homologous end-joining; Pichia pastoris.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Gene Deletion*
Genes, Fungal*
Homologous Recombination*
Pichia / genetics*