Potassium chloride released from contracting skeletal muscle may stimulate development of its hypertrophy

Irina V Kravchenko; Vladimir A Furalyov; Vladimir O Popov

doi:10.1016/j.bbrep.2019.100627

Potassium chloride released from contracting skeletal muscle may stimulate development of its hypertrophy

Biochem Biophys Rep. 2019 Mar 21:18:100627. doi: 10.1016/j.bbrep.2019.100627. eCollection 2019 Jul.

Authors

Irina V Kravchenko¹, Vladimir A Furalyov¹, Vladimir O Popov¹

Affiliation

¹ Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, Leninsky Ave. 33, Bld. 2, Moscow, 119071, Russia.

Abstract

The effects of potassium chloride on the expression of IGF-1 splice forms and myoblast proliferation were investigated. KCl at the concentrations of 7-12 mM stimulated the synthesis of IGF-1 and mechano growth factor (MGF) in murine myoblasts as well as in myotubes both at the mRNA and protein levels. Pan-calcium channel blocker CdCl₂ completely abolished stimulation of growth factor expression, whereas blocker of HCN and Na_v1.4 channels ZD7288 drastically reduced it. In addition, potassium chloride stimulated myoblast proliferation, while IGF-1 autocrine signaling inhibition partially suppressed these mitogenic effects.

Keywords: IGF-1; Mechano growth factor; Myoblasts; Myotubes; Proliferation.