Effect of radio frequency treatment on functional, structural and thermal behaviors of protein isolates in rice bran

Bo Ling; Shaohui Ouyang; Shaojin Wang

doi:10.1016/j.foodchem.2019.03.072

Effect of radio frequency treatment on functional, structural and thermal behaviors of protein isolates in rice bran

Food Chem. 2019 Aug 15:289:537-544. doi: 10.1016/j.foodchem.2019.03.072. Epub 2019 Mar 16.

Authors

Bo Ling¹, Shaohui Ouyang², Shaojin Wang³

Affiliations

¹ Northwest A&F University, College of Mechanical and Electronic Engineering, Yangling, Shaanxi 712100, China.
² Northwest A&F University, College of Food Science and Engineering, Yangling, Shaanxi 712100, China.
³ Northwest A&F University, College of Mechanical and Electronic Engineering, Yangling, Shaanxi 712100, China; Department of Biological Systems Engineering, Washington State University, 213 L.J. Smith Hall, Pullman, WA 99164-6120, USA. Electronic address: shaojinwang@nwsuaf.edu.cn.

PMID: 30955646
DOI: 10.1016/j.foodchem.2019.03.072

Abstract

To investigate the influence of radio frequency (RF) stabilization treatment on rice bran protein isolate (RBPI) properties, functional, structural and thermal behaviors of RBPI obtained from RF treated RB were evaluated under various temperatures between 80 and 120 °C holding for 5 min. The results showed that RF heating had negative impact on the yield, purity, foaming properties and solubility of RBPI when the treatment temperature was higher than 100 °C, while the absorption and emulsifying properties were improved. No significant changes were observed in primary structure of RF treated samples, but the second structure changed with an increase in random coil and a decrease in β-sheet, α-helix and β-turn. The decreased tryptophan fluorescence and increased surface hydrophobicity suggested alterations in tertiary structure. RF treatment also caused noticeable decrease in denaturation temperature and enthalpy of RBPI. The results would provide basic information for food applications of proteins isolated from RF stabilized RB.

Keywords: Functional properties; Radio frequency heating; Rice bran protein isolates; Structural analysis; Thermal behavior.

MeSH terms

Emulsions / chemistry
Heating
Hydrophobic and Hydrophilic Interactions
Oryza / chemistry*
Plant Proteins, Dietary / chemistry*
Protein Conformation, alpha-Helical
Protein Denaturation
Radio Waves
Solubility
Temperature

Substances

Emulsions
Plant Proteins, Dietary