Objective: To investigate the expression and significance of STOX1 in different stages of gestation villi and placenta. Methods: Totally 137 cases of normal villi and placenta of pregnant women were collected from the Department of Obstetrics of Shanghai Pudong Hospital from October 1(st) 2015 to February 28(th) 2018, including 64 cases of early pregnancy (early pregnancy group) which consists of 32 cases of 5-7(+6) weeks gestation (early pregnancy group A) and 32 cases of 8-11(+3) weeks gestation (early pregnancy group B), 28 cases of 14-26 weeks gestation(middle pregnancy group) and 45 cases of 37-41 weeks gestation (late pregnancy group). The expression and localization of STOX1 mRNA and protein in placenta were evaluated by RT-qPCR, Western blotting and immunohistochemistry. Results: (1)STOX1 was positively expressed in the cytotrophoblasts and syncytiotrophoblasts as well as interstitial and vascular endothelial cells of all groups. (2)STOX1 mRNA expression in each group was significantly different (P<0.05), the lowest was in the early pregnancy group A(0.007 8±0.000 4), which increased along with the progression of gestational age(P<0.05),and reached the highest level in the third trimester(0.064 4±0.001 3). (3)The protein level of STOX1 in different stages of normal pregnancy was 0.53±0.20 in early pregnancy group A;0.62±0.37 in early pregnancy group B;0.70±0.03 in middle pregnancy group and 0.81±0.04 in late pregnancy group respectively; which was positively related with the progression of gestational age (P<0.05). Conclusion: The expressions of STOX1 is gradually increasing along with the normal pregnancy progression, suggesting that it might be involved in proliferation, differentiation and infiltration and (or) apoptosis of trophoblast cells and the development of the placenta.
目的： 探讨STOX1在正常妊娠不同阶段绒毛及胎盘组织中的表达及其意义。 方法： 收集2015年10月1日至2018年2月28日在上海市浦东医院终止妊娠的64例正常早期妊娠者（孕早期组），其中包括32例妊娠5~7(+6)周（孕早A组）和32例妊娠8~11(+3)周（孕早B组），28例孕14~26周（孕中期组），以及45例孕37~41周（孕晚期组），共137例正常孕妇的绒毛和胎盘组织。采用实时荧光定量RT-qPCR技术、免疫印迹法和免疫组织化学SP法检测绒毛和胎盘组织STOX1基因和蛋白的表达差异及细胞定位。 结果： （1）在正常妊娠不同阶段的绒毛和胎盘组织中细胞滋养细胞、合体滋养细胞、血管内皮及绒毛间质中均可检测到STOX1蛋白的表达。（2）STOX1 mRNA的相对表达量各组间差异有统计学意义（P<0.05），早孕A组最低为（0.007 8±0.000 4），随着孕周进展升高，至孕晚期达最高水平（0.064 4±0.001 3）。（3）正常妊娠不同阶段的绒毛和胎盘组织中STOX1蛋白相对表达量分别为：孕早期A组0.53±0.20；孕早期B组0.62±0.37；孕中期组0.70±0.03；孕晚期组0.81±0.04；各组呈现为随着孕周的增加而上升趋势（P<0.05）。 结论： STOX1在正常妊娠进展过程中表达上调，提示STOX1可能参与了滋养细胞的增殖、分化、侵袭和（或）凋亡以及胎盘的发育。.
Keywords: Normal pregnancy; Placenta; STOX1; Trophoblast cells; Villus.