Study of target spacing of thermo-sensitive carrier on the activity recovery of immobilized penicillin G acylase

Ke Li; Guolei Shan; Xiaobing Ma; Xinyu Zhang; Zhenbin Chen; Zhenghua Tang; Zhen Liu

doi:10.1016/j.colsurfb.2019.03.064

Study of target spacing of thermo-sensitive carrier on the activity recovery of immobilized penicillin G acylase

Colloids Surf B Biointerfaces. 2019 Jul 1:179:153-160. doi: 10.1016/j.colsurfb.2019.03.064. Epub 2019 Apr 1.

Authors

Ke Li¹, Guolei Shan¹, Xiaobing Ma¹, Xinyu Zhang¹, Zhenbin Chen², Zhenghua Tang³, Zhen Liu⁴

Affiliations

¹ State Key Laboratory of Gansu Advanced Non-ferrous Metal Materials, Lanzhou University of Technology, Lanzhou, Gansu, 730050, China; School of Material Science and Engineering, Lanzhou University of Technology, Lanzhou, Gansu, 730050, China.
² State Key Laboratory of Gansu Advanced Non-ferrous Metal Materials, Lanzhou University of Technology, Lanzhou, Gansu, 730050, China; School of Material Science and Engineering, Lanzhou University of Technology, Lanzhou, Gansu, 730050, China. Electronic address: zhenbinchen@163.com.
³ Guangzhou Key Laboratory for Surface Chemistry of Energy Materials, New Energy Research Institute, School of Environment and Energy, South China University of Technology, Guangzhou Higher Education Mega Centre, Guangzhou, 5100067, China; Guangdong Provincial Key Laboratory of Atmospheric Environment and Pollution Control, Guangdong Provincial Engineering and Technology Research Center for Environmental Risk Prevention and Emergency Disposal, School of Environment and Energy, South China University of Technology, Guangzhou Higher Education Mega Centre, Guangzhou, 510006, China.
⁴ Department of Physics and Engineering, Frostburg State University, Maryland, 21532, United States. Electronic address: zhenliu8@gmail.com.

PMID: 30954878
DOI: 10.1016/j.colsurfb.2019.03.064

Abstract

The immobilized penicillin G acylase (PGA) is an important industrial catalyst, the activity recovery rate of it directly affects enterprise efficiency. How to improve the enzyme activity recovery rate has been a research focus in this field. Based on the above problems, this work further improved the activity recovery rate by adjusting the target spacing for the first time. Glycidyl methacrylate (GMA) was used as the immobilized target and methyl methacrylate (MMA) as the copolymer monomer. According to the copolymer composition equation of P(MMA-co-GMA), the thermo-sensitive copolymers, PDEA-b-PHEMA-b-P(MMA-co-GMA) with different target spacings, were synthesized rapidly and efficiently via reversible addition-fragmentation chain transfer (RAFT) polymerization method. The error range between the theoretical and actual values of MMA and GMA in the copolymers carrier was (0-4)%, which demonstrated that the reliability of using composition equation to accurately and quickly synthesize copolymers with specific spacing. Studies on the thermo-sensitive showed that the low critical solution temperature (LCST) of the copolymer carrier decreased with the increase of hydrophobic monomer. Most importantly, the activity recovery rate increased with the increase of target spacing, and when the molar ratio of MMA to GMA in the copolymer was 8.75:1, the recovery of activity of immobilized PGA could be up to 63.50%, which was 21.70% higher than that of pure GMA. This work provided an important idea for improving the activity of immobilized PGA.

Keywords: Activity recovery rate; Carrier; Penicillin G acylase; Reactivity ratio; Target spacing.

MeSH terms

Drug Carriers / chemistry*
Enzymes, Immobilized / metabolism*
Molecular Weight
Penicillin Amidase / metabolism*
Polymers / chemistry
Proton Magnetic Resonance Spectroscopy
Regression Analysis
Temperature*

Substances

Drug Carriers
Enzymes, Immobilized
Polymers
Penicillin Amidase