Bioluminescent aptamer-based solid-phase microassay to detect lung tumor cells in plasma

Eugenia E Bashmakova; Vasilisa V Krasitskaya; Galina S Zamay; Tatiana N Zamay; Ludmila A Frank

doi:10.1016/j.talanta.2019.03.030

Bioluminescent aptamer-based solid-phase microassay to detect lung tumor cells in plasma

Talanta. 2019 Jul 1:199:674-678. doi: 10.1016/j.talanta.2019.03.030. Epub 2019 Mar 5.

Authors

Eugenia E Bashmakova¹, Vasilisa V Krasitskaya², Galina S Zamay³, Tatiana N Zamay³, Ludmila A Frank⁴

Affiliations

¹ Institute of Biophysics SB RAS, Federal Research Center "Krasnoyarsk Science Center SB RAS, Akademgorodok 50/50, 660036 Krasnoyarsk, Russia; Siberian Federal University, Svobodny pr. 79, 660041 Krasnoyarsk, Russia.
² Institute of Biophysics SB RAS, Federal Research Center "Krasnoyarsk Science Center SB RAS, Akademgorodok 50/50, 660036 Krasnoyarsk, Russia.
³ State Medical University named after V.F. Voyno-Yasenetsky, Partizana Zheleznyaka St. 1, 660022 Krasnoyarsk, Russia.
⁴ Institute of Biophysics SB RAS, Federal Research Center "Krasnoyarsk Science Center SB RAS, Akademgorodok 50/50, 660036 Krasnoyarsk, Russia; Siberian Federal University, Svobodny pr. 79, 660041 Krasnoyarsk, Russia. Electronic address: lfrank@yandex.ru.

PMID: 30952314
DOI: 10.1016/j.talanta.2019.03.030

Abstract

Two high-affinity DNA aptamers for lung tumor cells were applied as biospecific elements in bioluminescent assay of patient blood. The oligonucleotide complementary to the 5' end of both aptamers carrying either biotin or Ca²⁺-regulated photoprotein obelin was used to form a sandwich-type analytical complex on the surfaces of magnetic streptavidin-activated microspherical particles. Clinical blood samples from cases of morphologically confirmed lung cancer and control samples were analyzed applying the developed assay. From the receiver operator curve (ROC) analysis, the chosen threshold value as clinical decision limit offers the sensitivity of 91.5% and the specificity of 75% (p < 0.001). The area under ROC curve with the value of 0.901 distinguishes well between the two groups under investigation.

Keywords: Bioluminescent solid-phase microassay; DNA aptamers; Lung tumor.

MeSH terms

Aptamers, Nucleotide / chemistry*
Humans
Luminescent Measurements*
Lung Neoplasms / blood*
Lung Neoplasms / diagnostic imaging*
Magnetic Fields
ROC Curve
Streptavidin / chemistry

Substances

Aptamers, Nucleotide
Streptavidin