A novel pyrimidine based deep-red fluorogenic probe for detecting hydrogen peroxide in Parkinson's disease models

Abstract

Parkinson's disease (PD) severely affects life quality of patients and has brought huge economic burden to health system worldwide. Previous studies have shown that the abnormal expression of hydrogen peroxide (H₂O₂) in the brain is closely related to the development of neurodegenerative diseases such as PD. Herein, we designed a novel deep-red H₂O₂ fluorogenic probe PB1 to detect the level of H₂O₂in vivo. PB1 showed a highly selectivity response to H₂O₂ over other reactants such as reactive oxygen/nitrogen species, biothiols and various ions in aqueous solution at physiological pH. We have demonstrated that PB1 possesses an excellent response to H₂O₂ in the cells and in the brain tissue of drosophila from confocal fluorescence imaging. These results suggested that PB1 holds great potential in the study of the relationship between H₂O₂ overexpression and PD.

Keywords: Cell and tissue imaging; Deep-red; Hydrogen peroxide; Parkinson's disease.