A Label-free aptasensor based on Aptamer/NH2 Janus particles for ultrasensitive electrochemical detection of Ochratoxin A

Ya-Juan Yang; Ying Zhou; Yang Xing; Guo-Mei Zhang; Yan Zhang; Cai-Hong Zhang; Peng Lei; Chuan Dong; Xu Deng; Yujian He; Shao-Min Shuang

doi:10.1016/j.talanta.2019.02.015

A Label-free aptasensor based on Aptamer/NH₂ Janus particles for ultrasensitive electrochemical detection of Ochratoxin A

Talanta. 2019 Jul 1:199:310-316. doi: 10.1016/j.talanta.2019.02.015. Epub 2019 Feb 11.

Authors

Ya-Juan Yang¹, Ying Zhou¹, Yang Xing¹, Guo-Mei Zhang¹, Yan Zhang¹, Cai-Hong Zhang¹, Peng Lei¹, Chuan Dong², Xu Deng³, Yujian He⁴, Shao-Min Shuang⁵

Affiliations

¹ School of Chemistry and Chemical Engineering, Shanxi University, Taiyuan 030006, China.
² Institute of Environmental Science, Shanxi University, Taiyuan 030006, China.
³ University of Electronic Science and Technology of China, Institute of Fundamental and Frontier Sciences, Chengdu 610054, Sichuan, China.
⁴ College of Chemistry and Chemical Engineering, University of Chinese Academy of Sciences, Beijing 100049, China. Electronic address: heyujian@ucas.ac.cn.
⁵ School of Chemistry and Chemical Engineering, Shanxi University, Taiyuan 030006, China. Electronic address: smshuang@sxu.edu.cn.

PMID: 30952263
DOI: 10.1016/j.talanta.2019.02.015

Abstract

In this study, a novel aptasensor based on Aptamer/NH₂ Janus particles is developed for the detection of Ochratoxin A(OTA). By coating gold on the hemispherical surface of the aminated polystyrene particles, Ochratoxin A aptamer is immobilized on the surface of the gold layer for selective identification and the other hemispherical able to bind to Glassy carbon electrode via peptide bond. Under optimum conditions, the sensor exhibited a wide dynamic range of OTA concentration from 1 × 10^-5 nM to 10 nM, and the detection limit is 3.3 × 10^-3 pM on condition of acceptable stability and reproducibility. The sensors were showed excellent performance in the detection of OTA in red wine sample with recoveries between 95.7% and 100.18%, which studied by the standard addition spiking technique. This work provides a new idea and method for preparing immune electrochemical sensors and is expected to be used for the OTA detection in red wine sample analysis.

Keywords: Electrochemical aptasensor; Janus Particles; OTA.

MeSH terms

Aptamers, Nucleotide / chemistry*
Biosensing Techniques*
Electrochemical Techniques*
Hydrogen-Ion Concentration
Ochratoxins / analysis*
Particle Size
Surface Properties
Time Factors

Substances

Aptamers, Nucleotide
Ochratoxins
ochratoxin A