A rod-shaped, endospore-forming, aerobic bacterium, designated FJAT-46582T, was isolated from a sediment sample of the coastal region in Xiapu County, Fujian Province in China. Growth was observed at 10-30 °C (optimum, 25 °C), in 0-7.0 % NaCl (0 %) and at pH 6.0-11.0 (pH 8.0), respectively. The cell-wall peptidoglycan contained meso-diaminopimelic acid and the isoprenoid quinone was MK-7. The main fatty acids were anteiso-C17 : 0 (26.5 %), anteiso-C15 : 0 (19.6 %), iso-C15 : 0 (14.4 %) and C16 : 0 (10.5 %). The main polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidyl ethanolamine. Phylogenetic analysis based on 16S rRNA gene sequences affiliated strain FJAT-46582T with the genus Bacillus, and showed the highest sequence similarity to Bacillus thermotolerans SGZ-8T (97.6 %) and Bacillus ectoinformans (97.1 %). The average nucleotide identity and in silico DNA-DNA hybridization values between strain FJAT-46582T and the most closely related species were 72.3 and 22.9 %, respectively, which were much lower than the thresholds commonly used to define species (96 and 70 %, respectively) indicating that it belonged to a different taxon. The DNA G+C content was 44.2 mol%. The phenotypic characters and taxono-genomics study revealed that strain FJAT-46582T represents a novel Bacillus species, for which the name Bacillus xiapuensis sp. nov. is proposed. The type strain is FJAT-46582T (=JCM 33155=CCTCC AB 2017047T).
Keywords: ANI; Bacillus xiapuensis sp. nov.; Fujian; Xiapu; coastal sediment; isDDH; taxono-genomics.