MHz data collection of a microcrystalline mixture of different jack bean proteins

Sci Data. 2019 Apr 3;6(1):18. doi: 10.1038/s41597-019-0010-0.

Abstract

We provide a detailed description of a serial femtosecond crystallography (SFX) dataset collected at the European X-ray free-electron laser facility (EuXFEL). The EuXFEL is the first high repetition rate XFEL delivering MHz X-ray pulse trains at 10 Hz. The short spacing (<1 µs) between pulses requires fast flowing microjets for sample injection and high frame rate detectors. A data set was recorded of a microcrystalline mixture of at least three different jack bean proteins (urease, concanavalin A, concanavalin B). A one megapixel Adaptive Gain Integrating Pixel Detector (AGIPD) was used which has not only a high frame rate but also a large dynamic range. This dataset is publicly available through the Coherent X-ray Imaging Data Bank (CXIDB) as a resource for algorithm development and for data analysis training for prospective XFEL users.

Publication types

  • Dataset
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Concanavalin A / chemistry*
  • Crystallization
  • Crystallography, X-Ray
  • Plant Proteins / chemistry*
  • Urease / chemistry*

Substances

  • Plant Proteins
  • Concanavalin A
  • concanavalin B protein, Canavalia ensiformis
  • Urease