Development of a multiplex-PCR to simultaneously detect acquired linezolid resistance genes cfr, optrA and poxtA in enterococci of clinical origin

Jennifer K Bender; Carola Fleige; Ingo Klare; Guido Werner

doi:10.1016/j.mimet.2019.03.025

Development of a multiplex-PCR to simultaneously detect acquired linezolid resistance genes cfr, optrA and poxtA in enterococci of clinical origin

J Microbiol Methods. 2019 May:160:101-103. doi: 10.1016/j.mimet.2019.03.025. Epub 2019 Mar 30.

Authors

Jennifer K Bender¹, Carola Fleige², Ingo Klare², Guido Werner²

Affiliations

¹ National Reference Centre for Staphylococci and Enterococci, Division of Nosocomial Pathogens and Antibiotic Resistances, Department of Infectious Diseases, Robert Koch Institute, Wernigerode, Germany. Electronic address: benderj@rki.de.
² National Reference Centre for Staphylococci and Enterococci, Division of Nosocomial Pathogens and Antibiotic Resistances, Department of Infectious Diseases, Robert Koch Institute, Wernigerode, Germany.

PMID: 30940534
DOI: 10.1016/j.mimet.2019.03.025

Abstract

Linezolid-resistant enterococcus spp. are increasingly recognized by diagnostic laboratories. Resistance can be mediated by the expression of cfr, optrA or poxtA. We developed a multiplex-PCR to simultaneously detect all three genes. The PCR is suitable for microbiological diagnostics in order to restrict further spread of resistances in enterococci.

Keywords: Enterococcus spp.; Linezolid resistance; Multiplex-PCR; cfr; optrA; poxtA.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

DNA, Bacterial / genetics
Drug Resistance, Bacterial / genetics*
Enterococcus faecalis* / genetics
Enterococcus faecalis* / isolation & purification
Enterococcus faecium* / genetics
Enterococcus faecium* / isolation & purification
Genes, Bacterial / genetics
Linezolid / therapeutic use
Multiplex Polymerase Chain Reaction / methods*
Plasmids / genetics

Substances

DNA, Bacterial
Linezolid