Interleukin gene polymorphisms in chronic periodontitis: A case-control study in the Indian population

Poulami Majumder; Suraj K Panda; Sujay Ghosh; Subrata Kumar Dey

doi:10.1016/j.archoralbio.2019.03.015

Interleukin gene polymorphisms in chronic periodontitis: A case-control study in the Indian population

Arch Oral Biol. 2019 May:101:156-164. doi: 10.1016/j.archoralbio.2019.03.015. Epub 2019 Mar 26.

Authors

Poulami Majumder¹, Suraj K Panda², Sujay Ghosh³, Subrata Kumar Dey²

Affiliations

¹ Department of Biotechnology, Centre for Genetic studies, School of Biotechnology and Biological Sciences, Maulana Abul Kalam Azad University of Technology, West Bengal, India. Electronic address: plm89.majumder@gmail.com.
² Department of Biotechnology, Centre for Genetic studies, School of Biotechnology and Biological Sciences, Maulana Abul Kalam Azad University of Technology, West Bengal, India.
³ Cytogenetics and Genomics Research Unit, Department of Zoology, University of Calcutta, West Bengal, India.

PMID: 30939298
DOI: 10.1016/j.archoralbio.2019.03.015

Abstract

Objective: The aim of the present study was to investigate the possible association between seven Interleukin (IL) gene polymorphisms and their interaction with the chronic inflammatory oral disease, chronic periodontitis in Indian population.

Design: A total of 357 study subjects (157 with chronic periodontitis and 200 with healthy control) were genotyped for IL1A -889C/T (rs1800587), IL1B -31C/T (rs1143627), IL1B -511A/G (rs16944), IL1B + 3954C/T (rs1143634), IL2 -330 T/G (rs2069762), IL4 -33C/T (rs2070874), IL6 -597 G/A (rs1800797), IL8 -251A/T (rs4073), IL10 -819C/T (rs1800871), IL10 -592A/C (rs1800872) and IL13 -1111C/T (rs1800925). Statistical analysis was performed using the statistical software package SPSS v16. SNPassoc and Multifactor Dimensionality Reduction algorithm v3.0.2.

Results: We found that the statistically significant association of IL1A-889C/T (rs1800587), IL1B -31C/T (rs1143627), IL1B -511A/G (rs16944) and IL1B + 3954C/T (rs1143634) gene polymorphisms with increased susceptibility of chronic periodontitis. The best gene-gene interaction model was IL1B(-31C/T) X IL1B(+3954C/T) X IL10(-819C/T) with 10/10 cross validation consistency. The variant allele of IL1A, IL1B, IL10 and IL13 were seemed to be linked with chronic periodontitis increased susceptibility. The results of this study also indicate that epidemiological factors especially oral habits also play an important role in the development of chronic periodontitis.

Conclusions: This study concludes IL1A -889C/T (rs1800587), IL1B -31C/T (rs1143627), IL1B -511A/G (rs16944), IL1B + 3954C/T (rs1143634), IL10 -819C/T (rs1800871), IL10 -592A/C (rs1800872) and IL13 -1111C/T (rs1800925) gene polymorphisms are significantly associated with chronic periodontitis. This work also infers that the best interactive model comprised of IL1B and IL10 polymorphisms cumulatively increase the risk of chronic periodontitis.

Keywords: Chronic periodontitis; Cross validation consistency; Genotype; Interleukin; Polymorphisms; Statistical analysis.

MeSH terms

Case-Control Studies
Chronic Periodontitis / genetics*
Genetic Predisposition to Disease
Genotype
Humans
India
Interleukins / genetics*
Polymorphism, Single Nucleotide

Substances

Interleukins