An orange calcium-modulated bioluminescent indicator for non-invasive activity imaging

Younghee Oh; Yunhee Park; Julia H Cho; Haodi Wu; Nicole K Paulk; Lan Xiang Liu; Namdoo Kim; Mark A Kay; Joseph C Wu; Michael Z Lin

doi:10.1038/s41589-019-0256-z

An orange calcium-modulated bioluminescent indicator for non-invasive activity imaging

Nat Chem Biol. 2019 May;15(5):433-436. doi: 10.1038/s41589-019-0256-z. Epub 2019 Apr 1.

Authors

Younghee Oh^{1

2

3

4}, Yunhee Park^{1

2

3}, Julia H Cho^{1

2

3}, Haodi Wu^{5

6

7}, Nicole K Paulk^{3

8

9}, Lan Xiang Liu^{1

2

3}, Namdoo Kim^{1

2

3

10}, Mark A Kay^{3

8}, Joseph C Wu^{5

6

7}, Michael Z Lin^{11

12

13}

Affiliations

¹ Department of Neurobiology, Stanford University, Stanford, CA, USA.
² Department of Bioengineering, Stanford University, Stanford, CA, USA.
³ Department of Pediatrics, Stanford University, Stanford, CA, USA.
⁴ Department of DNA analysis, National Forensic Service Seoul Institute, Yangcheon-gu, Seoul, Republic of Korea.
⁵ Department of Medicine, Stanford University, Stanford, CA, USA.
⁶ Department of Radiology, Stanford University, Stanford, CA, USA.
⁷ Stanford Cardiovascular Institute, Stanford University, Stanford, CA, USA.
⁸ Department of Genetics, Stanford University, Stanford, CA, USA.
⁹ Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, CA, USA.
¹⁰ Department of Chemistry, Kongju National University, Gongju, South Korea.
¹¹ Department of Neurobiology, Stanford University, Stanford, CA, USA. mzlin@stanford.edu.
¹² Department of Bioengineering, Stanford University, Stanford, CA, USA. mzlin@stanford.edu.
¹³ Department of Pediatrics, Stanford University, Stanford, CA, USA. mzlin@stanford.edu.

Abstract

Fluorescent indicators are used widely to visualize calcium dynamics downstream of membrane depolarization or G-protein-coupled receptor activation, but are poorly suited for non-invasive imaging in mammals. Here, we report a bright calcium-modulated bioluminescent indicator named Orange CaMBI (Orange Calcium-modulated Bioluminescent Indicator). Orange CaMBI reports calcium dynamics in single cells and, in the context of a transgenic mouse, reveals calcium oscillations in whole organs in an entirely non-invasive manner.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

MeSH terms

Animals
Calcium / chemistry*
Luminescent Measurements
Luminescent Proteins / chemistry*
Mice
Mice, Transgenic
Optical Imaging*
Organometallic Compounds / chemistry*

Substances

Luminescent Proteins
Organometallic Compounds
Calcium

Abstract

Publication types

MeSH terms

Substances

Grants and funding